Figure Legends
Figure 1. Susceptible AKR/J mice retain T. muris and have
a Type 1 immune skew. (A) Experimental schematic. (B) Worm burden in
AKR/J mice. Real time PCR analysis of proximal colon homogenate from
naïve and T. muris (Tm)-infected mice, quantifying (C)Ifng (D) Il4, (E) Il13 , (F) Fcer1a , and (G)Mcpt8 (normalized to Actb ). Data from all combined
experiments are presented as the mean ± SEM. Statistical analysis was
performed using a linear fixed-effect model with pairwise comparison; *P
≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. n=2-3 for each time
point per experiment; 3 independent experiments.
Figure 2. The basophil population expands upon T. murisinfection in AKR/J mice. Flow cytometric analysis for basophils in
naïve and T. muris (Tm)-infected mice. (A) Cecum basophil gating
(CD45+Lineage
(Lin)−FcεRIα+IgE+CD49b+ckit-).
Basophil percentage of (B) Lin- and (C)
CD45+ cecum cells. (D) Spleen basophil gating
(CD45+Lineage
(Lin)−FcεRIα+IgE+CD49b+ckit-).
Basophil percentage of (E) Lin- and (F)
CD45+ spleen cells. Data from all combined experiments
are presented as the mean ± SEM. Statistical analysis was performed
using a linear fixed-effect model with pairwise comparison; *P ≤ 0.05,
**P ≤ 0.01, ***P ≤ 0.001. n=2-3 for each time point per experiment; 3
independent experiments.
Figure 3. Basophil Notch2 expression does not substantially
increase following T. muris infection in AKR/J mice. Flow
cytometric analysis of basophils in the cecum in naïve and T.
muris (Tm)-infected mice, displaying (A) representative plots for
Notch2 staining, (B) percent Notch2+ basophils, and
(C) Notch2 geometric mean fluorescence intensity (gMFI) on cecum
basophils. Data from all combined experiments are presented as the mean
± SEM. n=2-3 for each time point per experiment; 3 independent
experiments.
Figure 4. Basophil Notch2 expression does not changes at day 14
post-T. muris infection upon IFN-γ neutralization in AKR/J mice.(A) Experimental schematic. IFN-γ protein levels as measured by ELISA in
(B) serum and (C) proximal colon tissue lysate, and (D) real time PCR
analysis of proximal colon homogenate quantifying Ifng(normalized to Actb ) from naïve and T. muris (Tm)-infected
mice. Flow cytometric analysis of basophils in the cecum in naïve and
Tm-infected mice, displaying (E) representative plots for Notch2
staining, (F) percent Notch2+ basophils, and (G)
Notch2 geometric mean fluorescence intensity (gMFI) on basophils. Data
from all combined experiments are presented as the mean ± SEM.
Statistical analysis was performed using a linear fixed-effect model
with pairwise comparison; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤
0.0001. n=2-3 for each time point per experiment; 3 independent
experiments.