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\begin{document}

\title{Development of disposable electrode for the detection of mosquito-borne
viruses}



\author[1]{Fahmida Nasrin}%
\author[1]{Indra Mwmdi Khoris}%
\author[2]{Ankan Dutta  Chowdhury}%
\author[1]{Sjakurrizal El  Muttaqein}%
\author[1]{Enoch Y. Park}%
\affil[1]{Shizuoka University}%
\affil[2]{Amity University - Kolkata Campus}%


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  \date{May 26, 2024}


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\begin{abstract}
Development of disposable, rapid and convenient biosensor with high
sensitivity and reliability is the most desired method of viral disease
prevention. To achieve this goal, in this work, a practical impedimetric
biosensor has been implemented into a disposable electrode on a
screen-printed carbon electrode (SPCE) for the detection of two
mosquito-borne viruses. The biosensor fabrication has step-wisely
carried out on the disposable electrode surface at room temperature:
starting from conductive film formation, physical binding of the gold
nanoparticles (AuNPs)-polyaniline (PAni) into the conductive film, and
biofunctionalization. To get the maximum efficiency of the antibody,
biotinylated antibody has been conjugated on the surface of AuNP-PAni
/PAni-SPCE via the streptavidin-biotin conjugation method which is a
critical factor for the high sensitivity. Using the antibody-antigen
interaction, this disposable electrode has designed to detect
mosquito-borne infectious viruses, Chikungunya virus (CHIKV) and Zika
Virus (ZIKV) separately in a wide linear range of 100 fg/mL to 1 ng/mL
with a low detection limit of 1.33 fg/mL and 12.31 fg/mL, respectively.%
\end{abstract}%



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