Sequencing and Alignment
PCR products were kept in PCR plates sealed with adhesive film or caps and sent oversea via French post services to be sequenced. Sanger sequencing was handled by Microsynth facility in Lyon, France. Forward and reverse sequencing were realized with the same set of primers than for the PCR reaction. Fragment sequences were aligned using BLAST (NCBI, NIH) against the standard databases (nucleotide collection nr/nt)[40,41] and Anopheles species identifications were retrieved for forward and reverse sequences individually and integrated in our data and metadata files.
Reference sequences of ITS2 region of 14 Anopheles species found in French Guiana are available online on the NIH website (PopSet: 870902931) [31] .