Agarose Gel
1 % agarose gel was made with 1 g UltraPure Agarose (Invitrogen), 100
mL 0.5X TBE Buffer diluted from TBE Buffer 10X (Biosolve) and 10 µL
Midori Green Advance (Nippon Genetics). 8 µL of each sample was mixed
with 2 µL DNA Gel Loading Dye 6X (Thermo Scientific) and 10 µL of 100 bp
DNA Ladder Ready to Load size marker (Solis Biodyne) was used as
reference. Migration took 45 min at 120 V with Owl EC300XL Compact Power
Supply (Thermo Scientific). Gel visualization and analysis were done
using Alliance Q9 Advanced imaging system and software (Uvitec
Cambridge).