Figure 4 NKG2D regulates differentiation of CD4+ T
cells. Genetically modified CD4+ T cells transfected
with LV-NKG2D, V-siRNA and LV-BC from juvenile idiopathic arthritis
(JIA) patients and healthy controls were co-cultured with irradiated DCs
at the rate of 3:1 with anti-CD3 and interleukin (IL)-15 for 3 days. (A)
Production of transforming growth factor-beta (TGF-β), IL-10 and IL-12
in the supernatant was determined by ELISA. (B and C) Subsets of
CD25+Foxp3+CD4+,
IL-17+CD4+, interferon-gamma
(IFN-γ)+CD4+, and
IL-4+CD4+ T cells assayed by flow
cytometry. (D) Relative expression of mRNA relative of RORc, Foxp3,
T-bet and GATA3 transcription factors detected by real-time quantitative
RT-PCR (n = 6 each group). * P < 0.01 and # P <
0.05 for within group interaction.