ELISA detection of cytokines and NKG2D ligands
Serum soluble MICA (sMICA) and soluble MICB (sMICB) from JIA patients
and health control serum were analyzed using ELISA kits (R & D Systems,
USA). After cell culture and collection of cells, the supernatants were
harvested and tested for TGF-β, IL-10, IL-12p70 using ELISA kits
(Biolegend). The assays were performed according to the manufacturer’s
instructions in duplicates. Background values were determined and served
as control values. Optical densities were read at 450 nm, and the
concentrations of cytokines in each sample were determined from the
standard curves.