1.2 CRISPR Cas9 screening
Currently, three different categories of functional gene screening
libraries are offered: cDNA libraries, RNA
interference libraries, and
CRISPR Cas9 gene editing libraries. The latter is further classified
into CRISPR-Cas9 knockout libraries, CRISPR-dCas9 activation libraries,
and CRISPR-dCas9 interference libraries 17, with the
gene-scale CRISPR-Cas9 knockout (GeCKO) 18 being the
most prevalent CRISPR library screen. As shown in Figure 1, GeCKO
accomplishes this through a series of processes to achieve the screening
of genes 19 and drug sites of action18and the identification of the mechanism of action of
various impact factors20.