Material and Method
Formalin-fixed-paraffin-embedded H&E sections were made from the
radical parotidectomy specimen in the usual manner. Immunostainings for
AE1/3, p16, p40, S100 and Ki67 were performed using the Dako Omnis and
Leica Bond-III automated IHC
systems, immunostaining for CD1a used the Leica Bond-III automated IHC
system.
Representative sections were subjected to CSF1 break-apart FISH test
with FISH probes targeting the 5’end and 3’end of the CSF1 locus labeled
orange and green respectively (Empire Genomics). Any fusion transcripts
of the specimen were studied by Illumina RNA pan-cancer RNA-seq panel
(Illumina), which covered the fusion transcripts of 1385 cancer-related
genes including CSF1 gene. The expression of CSF1 gene was detected
by RNAscope™ Probe- Hs-CSF1 (Advanced Cell Diagnostics).