Figure legends:
Figure 1. Bone marrow aspirate, biopsy and flow cytometry at diagnosis showing atypical APL morphology and immunophenotype. a) morphologic review of bone marrow aspirate showing heavy infiltration by bilobed promyelocytes (wright-giemsa stain ×500, see arrow); b) abnormal promyelocyte with Auer rods, (wright-giemsa stain ×500, see arrow); c) bone marrow biopsy showing hypercellular marrow infiltrated by sheets of promyelocytes ( H&E ×40); d) flow cytometry of bone marrow aspirate, illustrating a blast population positive for CD45 with an intermediate to high side scatter; e) CD34+ (partial); f) CD56+ ( dim) and CD2 positive flow.
Figure 2. Molecular genetic analyses by PCR showing PML-RARα and FLT3-ITD detection. qPCR data obtained from amplification ofPML-RARα isoforms bcr3 (a, upper panel) and bcr1 (b, middle panel) at diagnosis on RotorGene instrument using commercially available kits (Ipsogen Qiagen, Germany). The graph shows a high positive (***), low positive (*) and abl1 gene (c, lower panel) as internal control used in each qPCR run amplification curves; d) PCR followed by gel electrophoresis for the detection of FLT3-ITD using commercially available kit (invivoscribe, USA). Lanes are marked as: ladder, 100 bp marker; water control; PC positive control for FLT3-ITD; NC wild type FLT3; #1 and #2 (our case) patient specimen. The expected size range is marked between 300-400 bp.