Figure legends:
Figure 1. Bone marrow aspirate, biopsy and flow cytometry at
diagnosis showing atypical APL morphology and immunophenotype. a)
morphologic review of bone marrow aspirate showing heavy infiltration by
bilobed promyelocytes (wright-giemsa stain ×500, see arrow); b) abnormal
promyelocyte with Auer rods, (wright-giemsa stain ×500, see arrow); c)
bone marrow biopsy showing hypercellular marrow infiltrated by sheets of
promyelocytes ( H&E ×40); d) flow cytometry of bone marrow aspirate,
illustrating a blast population positive for CD45 with an intermediate
to high side scatter; e) CD34+ (partial); f) CD56+ ( dim) and CD2
positive flow.
Figure 2. Molecular genetic analyses by PCR showing PML-RARα and
FLT3-ITD detection. qPCR data obtained from amplification ofPML-RARα isoforms bcr3 (a, upper panel) and bcr1 (b, middle
panel) at diagnosis on RotorGene instrument using commercially available
kits (Ipsogen Qiagen, Germany). The graph shows a high positive (***),
low positive (*) and abl1 gene (c, lower panel) as internal control used
in each qPCR run amplification curves; d) PCR followed by gel
electrophoresis for the detection of FLT3-ITD using commercially
available kit (invivoscribe, USA). Lanes are marked as: ladder, 100 bp
marker; water control; PC positive control for FLT3-ITD; NC wild type
FLT3; #1 and #2 (our case) patient specimen. The expected size range
is marked between 300-400 bp.