Figure 1: “Non-toxic” S. aureus survive better in
macrophages than CoNS.
After phagocytosis and lysostaphin/gentamicin treatment for 1 h (t0)
THP-1-CWT cells were further incubated for 24 h in medium containing
gentamicin to kill extracellular/escaped bacteria. At indicated time
points, the cells were lysed and bacterial CFU determined (A ).
Membrane integrity of the THP-1- CWT cells were assessed by LDH-assay
(B ).
Bacteria containing macrophages were enumerated by live/death staining.
Cells were seeded in an IBIDI-slide (C ). Quantification of
Syto9 positive macrophages (D ). Quantification of Syto9
positive bacteria in positive macrophages (E ).
The data represent at least three biological replicates. Significance
was determined by one-way analysis of variance with Tukey‘s multiple
comparison post-test (panel A, B, D ) or students t-test for
panel E .