Live-Imaging
For Live-cell Microscopy cells were infected with bacteria
constitutively expressing mCherry. THP1-CWT cells were seeded in a 4
Compartment Cell Culture Dish (Greiner) and infection was performed like
already described. Live-Imaging was started at t0 with a LSM800
microscope (Zeiss) using a 40 x objective with immersion oil. The yellow
fluorescent protein (YFP) channel received an excitation wavelength of
488 nm, an emission wavelength of 509 nm and a detection wavelength of
490-575 nm. For detection of mCherry, an excitation wavelength of
587 nm, an emission wavelength of 610 nm and a detection wavelength of
565-700 nm were used.