Figure 4: Intracellular acidification is linked to killing of CoNS but not non-toxic S. aureus.
After phagocytosis and lysostaphin/gentamicin treatment for 1 h (t0) THP-1-CWT cells were further incubated for 3 h or 24 h in medium containing gentamicin to kill extracellular/escaped bacteria. At indicated time points, the cells were lysed and bacterial CFU determined (A ). Membrane integrity of the THP-1, CWT cells were assessed by LDH-assay (B ).
The data represent at least three biological replicates. Significance was determined by one-way analysis of variance with Tukey‘s multiple comparison post-test.