Annotation of candidate loci and gene ontology
Of the 213 candidate loci, 209 were successfully aligned to the M.
ochrogaster genome. Eight loci were located in exons, 86 in introns,
and 115 in intergenic regions. We identified 74 genes associated with
the candidate loci, some genes contained several loci (Table S5).
Several of these genes were associated with functions in lipid
metabolism, energy homeostasis, and immunity (Table 2). Among GO terms
associated with the genes, six were significantly enriched in our data
set, including ”regulation of respiratory burst” and ”dicarboxylic acid
transport” (Table S6).
Table 2. Candidate genes detected by at least two genome scan methods,
and associated with lipid metabolism, energy homeostasis and immunity.
Locus refers to the location on the M. ochrogaster reference
genome (Chromosome_base-position). Method of outlier detection (L:
LFMM, Ba: Bayenv2, RDA: Redundancy Analysis)