Figure 5. Homing and survival of Treg in NSG mice. (A ) IVIS images of NSG mice injected with Treg generated with the StM of the IL-7M at time 0 (pre-injection), 12 hours and 48 hours after Treg injections. (B ) FACS plots showing the expression of CXCR4, CCR7, CCR2 and CCR9 on Treg expanded with the StM (green line) of the IL-7M (blue line). (C ) FACS plot showing the presence of HLA-A*0201+ Treg (feeder PBMC were HLA-A*0201-) in NSG mice at day 0 (pre-transfer), 2, 3, 5, 7, 9, and 11 (upper plots). Gated HLA-A*0201+Treg were detected according to labeling with CFSE (Treg StM) and PBSE (Treg IL-7M) (lower plots). (D ) Graph shows the kinetic of Treg in NSG mice over an 11 day observation period. (E ) Detection of Treg generated with the StM of the IL-7M at day 14 after transfer in the spleen of NSG mice.