Statistical analysis
Statistical analyses were performed using GraphPad Prism software. Flow
cytometry data were calculated as median fluorescence intensity (MFI)
and subpopulations as percentage of positive cells. Proliferation was
calculated as percentage of cells that diluted the fluorescent dye CFSE
(%CFSEdim). Data were presented as median and interquartile range
(IQR), and the Wilcoxon matched-pairs test was used for comparisons. A
two-tailed P value of 0.05 was considered significant.