T-Cell Bio-energetic Profile
Surrogate markers of oxidative phosphorylation and glycolysis were measured by flow cytometry using the following reagents according to the manufacturer’s instructions. MitoTracker Green FM and MitoTracker Red FM were from Invitrogen-Molecular Probes. 2-(N-(7-nitrobenz-2-oxa-,1,3-diazol-4-yl)amino)-2-deoxyglucose (2NBDG) was from Life Technologies. L(+)lactate concentration was measured in the supernatants with a Lactate Assay Kit (Sigma-Aldrich).