Figure 3. Expansion of Treg in the presence of IL-7. (A) Fold expansion of naïve Treg after 14 days culture with the StM (aCD3CD28 beads + 100UI/ml rh IL-2) and the IL-7M (aCD3CD28 beads + 100UI/ml rh IL-2+ 10ng/ml rhIL-7) (n=9) (B ) Representative FACS plots showing the phenotype of Treg after expansion with the StM or the IL-7M. (C ) Graph shows the percentage of Treg subsets after 14 days expansion with the StM or the IL-7M and at day 17 after resting (medium only) (n=8) (D ) Suppression assay of Treg after expansion with the StM or the IL-7M after 14 days expansion and at day 17 after resting (medium only) (n=3). (E ) CFSE dilution assay showing the number of cell cycles performed by Treg in the first 5 days of expansion with the StM or the IL-7M (F ) Graph shows the percentage of cells in each cycle after 5 days. (G) Graph shows the percentage of cells displaying T naïve (Tn, CD45RA+CD62L+ CD95-), T stem cell memory (Tscm, CD45RA+ CD62L+CD95+), T central memory (Tcm, CD45RA-CD62L+CD95+), T effector memory (tem, CD45RA-CD62L- CD95+) phenotype.