Western Blotting
Total protein was extracted from the PVs, SVC, RA, and LA. Western blot
was performed as previously documented. 11 The
membranes were incubated with the primary antibody
KCa3.1 (1:10000, Bioss, USA) overnight at 4 °C. Then,
they were then washed three times and were incubated with the secondary
antibody for 2 hours at room temperature. The protein levels were
normalized to those of GADPH (1:5000, Abcam, USA). The blot images were
scanned and analyzed using image analyzer software (AlphaEase FC, USA).