Western Blotting
Total protein was extracted from the PVs, SVC, RA, and LA. Western blot was performed as previously documented. 11 The membranes were incubated with the primary antibody KCa3.1 (1:10000, Bioss, USA) overnight at 4 °C. Then, they were then washed three times and were incubated with the secondary antibody for 2 hours at room temperature. The protein levels were normalized to those of GADPH (1:5000, Abcam, USA). The blot images were scanned and analyzed using image analyzer software (AlphaEase FC, USA).