Differential Densities of KCa3.1 in rabbit thoracic veins and atrium
Figure 2A shows a typical example of KCa3.1 distribution in cardiomyocytes in diverse cardiac preparations such as PVs, SVC, RA, and LA. As shown in Figure 2B, the expression of KCa3.1 was significantly higher in the PVs than in the RA and LA (8.61±6.68 vs 4.57±5.19; P<0.001 8.61±6.68 vs 3.20±1.99; P<0.001), and the expression of KCa3.1 in the PVs was significantly higher than SVC (7.20±3.90 vs 8.61±6.68; P<0.001). The expression of KCa3.1 in the SVC was significantly higher than RA and LA (7.20±3.90 vs 4.57±5.19; P<0.001 7.20±3.90 vs 3.20±1.99; P<0.001). The expression of KCa3.1 in the RA was significantly higher than LA (4.57±5.19 vs 3.20±1.99; P < 0.001).
As shown in Figure 3, western blotting also shows that KCa3.1 is more selectively expressed in thoracic veins than in the atrium. The expression of KCa3.1 was significantly higher in the PVs than in the RA and LA (0.73±0.01 vs RA:0.25±0.02; P<0.001 0.73±0.01 vs 0.22±0.01; P<0.001), and the expression of KCa3.1 in the PVs was significantly higher than SVC (0.73±0.01 vs 0.69±0.02; P<0.001). The expression of KCa3.1 in the SVC was significantly higher than RA and LA (0.69±0.02 vs 0.25±0.02; P<0.001 0.69±0.02 vs 0.25±0.02; P<0.001). The expression of KCa3.1 in the RA was significantly higher than LA (0.25±0.02vs 0.22±0.01; P < 0.05).