Expression and purification of recombinant BsAbs and cynomolgus
monkey FcRn
The BsAbs used in this study were expressed in either transient HEK-293
or stably-transfected Chinese hamster ovary (CHO) cells and purified
using conventional antibody purification processes to be
>95% purity.
Recombinant, soluble cynomolgus monkey FcRn was expressed in 293EBNA
cells transfected with the plasmids encoding for the soluble portion
FcRn and β2m and the protein was purified as previously
described.24, 25