Differential Scanning Calorimetry Analysis
Thermal stability of the BsAbs was assessed by DSC. Samples were
prepared at 1 mg/mL in PBS. Thermal transition of each antibody was
measured using an automated MicroCal PEAQ-DSC (Malvern Panalytical, UK)
in a scan rate of 1°C/min for the temperature range of 20-110°C, after
equilibration at 20°C for 3-min. Thermograms for each sample were buffer
referenced and baseline subtracted.