Expression and purification of recombinant BsAbs and cynomolgus monkey FcRn
The BsAbs used in this study were expressed in either transient HEK-293 or stably-transfected Chinese hamster ovary (CHO) cells and purified using conventional antibody purification processes to be >95% purity.
Recombinant, soluble cynomolgus monkey FcRn was expressed in 293EBNA cells transfected with the plasmids encoding for the soluble portion FcRn and β2m and the protein was purified as previously described.24, 25