Figure 3. Treatments with CP11 increase the number of
motoneurons whose axons have regenerated successfully. A. Image of a
horizontal section through the right side of the lumbar spinal cord of a
mouse in which the sciatic nerve was cut and repaired four weeks
earlier. Motoneurons were labeled by application of different
fluorescent retrograde tracers into the reinnervated gastrocnemius
(GAST, red) and tibialis anterior (TA, green) muscles. Three neurons in
this image contained both tracers (yellow arrows). This mouse was
treated with intraperitoneal injections of CP11. B. Mean (+ SEM)
number of motoneurons retrogradely labeled from tracer injection into
GAST and TA four weeks after sciatic nerve transection and repair and
two weeks after treatment, either by intraperitoneal injection (i.p.) or
orally (oral), with compound 11 (CP11, 10 mg/Kg), or vehicle. Numeric
values indicate p values from pairwise comparisons (ns = not
significant).
Figure 4. Treatments with compound 11 (CP11) increased the
number of muscle sensory neurons whose axons regenerated successfully.
A. In a section from the L4 DRG of a mouse four weeks after sciatic
nerve transection and repair and two weeks after treatment with CP11,
neurons retrogradely labeled from tracer injections into GAST (red) and
TA (green) are shown. Three cells in this image were labeled by both
tracers (yellow arrows). B. Mean (+ SEM) numbers of L4 DRG neurons
retrogradely labeled from TA, GAST, or Both in vehicle-treated and
CP11-treated mice are shown. Each symbol in each graph represents the
number of labeled neurons in a single animal in the series. Significance
of differences between groups are displayed as p values above comparison
brackets.