2. Methods
All animal care and experimental procedures were reviewed and approved
by the institutional animal welfare and ethical review body (AWERB) at
the University of Warwick or in accordance with the National Institutes
of Health Guidelines for the Use of Animals. Experiments were performed
under the appropriate project licenses with local and national ethical
approval. Animals were kept in standard housing with littermates,
provided with food and water ad libitum and maintained on a 12:12
(light-dark) cycle. The ArcKR knock-in mice were originally produced by
the Ingenious Targeting Laboratory (Ronkonkoma, NY). Gene targeting was
performed in iTL IC1 (C57BL/6) ES cells to introduce 2-point mutations
within Exon 1 of the Arc gene that when encoded substitutes
lysine to arginine at positions 268 and 269
(Wall et al. , 2018).