Figure 2. The amplitude of long-term potentiation (LTP) induced
by theta burst stimulation is significantly reduced in ArcKR compared to
WT mice.
A, Graph plotting mean normalised (to the baseline) fEPSP slope
against time for WT (n = 13 slices, 11 mice) and ArcKR mice
(n = 13 slices, 8 mice). After a 20-minute baseline, LTP was
induced with theta burst stimulation (TBS, 4 trains separated by 20 s,
with each train consisting of 10 bursts of 4 pulses at 100 Hz, separated
by 200 ms). Inset, example fEPSP waveforms before and after LTP
induction (average of waveforms at 115-120 minutes) from WT and ArcKR
mice. B, Bar chart plotting the mean potentiation of fEPSP slope 5
minutes post LTP induction (short term potentiation; STP). There was no
significant difference between the STP produced in slices from ArcKR and
from WT mice (mean STP: WT 117 ± 20 %; ArcKR 69.7 ± 7.3 %, P = 0.054,
Z = -1.923, U = 47, Mann Whitney). C, Bar chart plotting the mean
potentiation of fEPSP slope 115-120 minutes post TBS (LTP). The
magnitude of LTP was significantly reduced in slices from ArcKR vs WT
mice (mean LTP: WT 113.4 ± 28.8 %; ArcKR 48.7 ± 6.6 %, P = 0.013, Z =
-2.487, U = 36, Mann Whitney). Data are represented as mean ± SEM. The
data points in B and C are from single slices.