Figure 2. The amplitude of long-term potentiation (LTP) induced by theta burst stimulation is significantly reduced in ArcKR compared to WT mice.
A, Graph plotting mean normalised (to the baseline) fEPSP slope against time for WT (n = 13 slices, 11 mice) and ArcKR mice (n = 13 slices, 8 mice). After a 20-minute baseline, LTP was induced with theta burst stimulation (TBS, 4 trains separated by 20 s, with each train consisting of 10 bursts of 4 pulses at 100 Hz, separated by 200 ms). Inset, example fEPSP waveforms before and after LTP induction (average of waveforms at 115-120 minutes) from WT and ArcKR mice. B, Bar chart plotting the mean potentiation of fEPSP slope 5 minutes post LTP induction (short term potentiation; STP). There was no significant difference between the STP produced in slices from ArcKR and from WT mice (mean STP: WT 117 ± 20 %; ArcKR 69.7 ± 7.3 %, P = 0.054, Z = -1.923, U = 47, Mann Whitney). C, Bar chart plotting the mean potentiation of fEPSP slope 115-120 minutes post TBS (LTP). The magnitude of LTP was significantly reduced in slices from ArcKR vs WT mice (mean LTP: WT 113.4 ± 28.8 %; ArcKR 48.7 ± 6.6 %, P = 0.013, Z = -2.487, U = 36, Mann Whitney). Data are represented as mean ± SEM. The data points in B and C are from single slices.