Tables and Figures

Table 1. Primers for the quantification of total abundance of bacterial 16S rRNA genes and fungal ITS2 region, mcr A (methyl coenzyme M reductase A: methanogens), pmo A (particulate methane monooxygenase: methanotrophs), nif H (nitrogenase: N fixers),nos Z (nitrous oxide reductase: N2O reducers), archaeal amo A and bacterial amo A (archaeal and bacterial ammonia monooxygenase: ammonia oxidizers), nir S and nir K (nitrite reductase: nitrite reducers), nxr B (beta subunit of nitrite oxidoreductase: nitrite oxidizers), nor B (nitric oxide reductase: N2O producers) and pho D (alkaline phosphatase D: organic phosphorus hydrolyzers) by qPCR. The top six genes (bold fonts) were compared with both synthetic DNA standard and plasmid DNA standard.