Fig. 2. Amplification curves of each target gene by
serial dilutions between
synthetic DNA (column A) and plasmid DNA (column B) standards.Standards were diluted by 10 times for each step from
108 to 101 copies per μl. NC:
negative control. Rn is the fluorescence of the reporter dye divided by
the fluorescence of a passive reference dye; i.e., Rn is the reporter
signal normalized to the fluorescence signal of Applied Biosystems™ ROX™
Dye.