ROS analysis in Camelina seedlings
ROS was analyzed in Camelina WT and CsGGCT2;1 OE lines grown on ½
x MS and AsIII treatments using H2DCF-DA staining. The
seedlings were grown for two weeks on the media, and the root tips were
stained with H2DCF-DA, washed with DI water, and then
observed under confocal fluorescent microscopy. As depicted by
fluorescence, WT roots had significantly higher ROS under AsIII
treatments as compared to the CsGGCT2;1 OE lines (Figure 4c).
Based on quantification of fluorescence, there was a significant
(~ 40%) reduction in fluorescence in CsGGCT2;1OE lines than in the WT plants (Figure 4d).