Analysis of chlorophyll content and lipid peroxidation
Chlorophyll content was measured in Camelina WT and CsGGCT2;1 OE
lines exposed to control and AsIII treatments. There were no significant
differences between WT and OE lines under control conditions. However,
under the AsIII treatment, all the CsGGCT2;1 OE plants had leaves
that were darker green in color and had higher chlorophyll content than
the WT plants (Figure 4a). CsGGCT2;1 OE lines #9, #102, and
#103 had 70%, 123%, and 115%, respectively, higher chlorophyll than
the WT plants.
MDA levels as an indication of membrane damage due to lipid peroxidation
were measured under normal and AsIII stress conditions. Like biomass and
chlorophyll content, WT and CsGGCT2;1 OE lines had no significant
difference in lipid peroxidation under control conditions. However, WT
plants had significantly higher MDA content than CsGGCT2;1 OE
lines under AsIII treatment (Figure 4b). In response to AsIII stress,
the CsGGCT2;1 OE lines had approximately 30% lesser MDA than WT,
indicating that CsGGCT2;1 prevents membrane damage due to
AsIII-induced oxidative stress.