Fig. 1. Effects of brief EE exposure on medial prefrontal cortex protein levels.
Quantification of vGluT1 and GLT-1, of NMDA receptor subunits, GluN1, GluN2A, and GluN2B, of the AMPA receptor subunits, GluA1 and GluA2, of scaffolding proteins, SAP102, SAP97, GRIP, and PSD95, and the cytoskeletal marker Arc/Arg3.1 in the post-synaptic density (a)and in the whole homogenate (b) of the medial prefrontal cortex (mPFC) 2 hours after the end of EE exposure. In the right insert, representative immunoblots are shown for vGluT1 (62 kDa), GLT-1 (60 kDa), GluN1 (120 kDa), GluN2A (180 kDa), GluN2B (180 kDa), SAP102 (102 kDa), GluA1 (108 kDa), GluA2 (108 kDa), SAP97 (97 kDa), GRIP (130 kDa) Arc/Arg3.1 (55 kDa) and β-actin (43 kDa) in the post-synaptic density or the homogenate of mPFC. Data are shown as the mean + SEM and are expressed as percentage of the NoEE group. NoEE = white columns; EE = teal columns. N = 6 rats/group. *p < 0.05,**p < 0.01, ***p < 0.001 vs. NoEE; unpaired two-tailed Student’s t-test or Mann-Whitney test.
Fig. 2. Effects of brief EE exposure on nucleus accumbens protein levels .
Quantification of vGluT1 and GLT1, of NMDA receptor subunits, GluN1, GluN2A, and GluN2B, of the AMPA receptor subunits, GluA1 and GluA2, of scaffolding proteins, SAP102, SAP97, GRIP, and PSD95, and the cytoskeletal marker Arc/Arg3.1 in the post-synaptic density (a)and in the whole homogenate (b) of nucleus accumbens (NAc) 2 hours after the end of EE exposure. In the right insert, representative immunoblots are shown for vGluT1 (62 kDa), GLT-1 (60 kDa), GluN1 (120 kDa), GluN2A (180 kDa), GluN2B (180 kDa), SAP102 (102 kDa), GluA1 (108 kDa), GluA2 (108 kDa), SAP97 (97 kDa), GRIP (130 kDa) Arc/Arg3.1 (55 kDa) and β-actin (43 kDa) in the post-synaptic density or the homogenate of NAc. Data are shown as the mean + SEM and are expressed as percentage of the NoEE group. NoEE = white columns; EE = teal columns. N = 6 rats/group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. NoEE; unpaired two-tailed Student’s t-test or Mann-Whitney test.
Fig. 3. Effects of brief EE exposure on hippocampus protein levels.
Quantification of vGluT1 and GLT-1, of NMDA receptor subunits, GluN1, GluN2A, and GluN2B, of the AMPA receptor subunits, GluA1 and GluA2, of scaffolding proteins, SAP102, SAP97, GRIP, and PSD95, and the cytoskeletal marker Arc/Arg3.1 in the post-synaptic density (a)and in the whole homogenate (b) of hippocampus (Hipp) 2 hours after the end of EE exposure. In the right insert, representative immunoblots are shown for vGluT1 (62 kDa), GLT-1 (60 kDa), GluN1 (120 kDa), GluN2A (180 kDa), GluN2B (180 kDa), SAP102 (102 kDa), GluA1 (108 kDa), GluA2 (108 kDa), SAP97 (97 kDa), GRIP (130 kDa) Arc/Arg3.1 (55 kDa) and β-actin (43 kDa) in the post-synaptic density or the homogenate of Hipp. Data are shown as the mean + SEM and are expressed as percentage of the NoEE group. NoEE = white columns; EE = teal columns. N = 6 rats/group. *p < 0.05,**p < 0.01, ***p < 0.001 vs. NoEE; unpaired two-tailed Student’s t-test or Mann-Whitney test.