Mapping and annotation of GO terms
Annotation steps were performed in OmicsBox version 1.3 (https://www.biobam.com/omicsbox/). We utilized AUGUSTUS to performab initio gene prediction (Stanke & Morgenstern, 2005). Identified gene sequences were submitted to functional annotation usingblastp against a vast eukaryote database (2578 taxa) that included non-redundant GenBank CDS translations, PDB, SwissProt, PIR, and PRF but excluding environmental samples from whole-genome-sequence projects. The top ten hits below an e-value threshold of 10e5 were kept. Gene ontology (GO) terms mapping and annotation was complemented with scans of identified gene sequences with the InterPro database to obtain information about protein families, domains, and special features (Paysan-Lafosse et al., 2022).