Strains and medium
All strains used in this study are compiled in Table 1 .S. cerevisiae BTCC3 (haploid) was obtained from Indonesian
Culture Collection (InaCC, Cibinong Science Center, BRIN, Indonesia).
This strain and its derivatives were cultivated using a
yeast/peptone/dextrose (YPD) medium (20 g∙L-1 Bacto
peptone [BD, San Jose, CA], 10 g∙L-1 Bacto yeast
extract [BD], and 50 g∙L-1 glucose [BD]).Escherichia coli JM109 (Takara Bio, Shiga, Japan) was used for
cloning. Luria-Bertani medium (10 g∙L-1 tryptone
[Nacalai, Japan], 5 g∙L-1 yeast extract [BD],
and 10 g∙L-1 sodium chloride [Nacalai tesque Inc.,
Japan]) was used for cultivation.