Analysis of fermentation products
The medium broth was periodically sampled. After centrifugation at10,000
rpm for 5 min, the supernatant was filtered through a 0.45-μM
syringeless PTFE filter. Concentrations of glucose and other
fermentation products were determined via High Performance Liquid
Chromatography (HPLC) (LC-20AB, Shimadzu, Japan) using a Coregel-87H3
column (7.8 mm, I.D. x 300 mm Transgenomic) at 80 °C for 40 min using 5
mM of sulfuric acid as eluent (flow rate at 0.6
mL∙min-1). A refractive index detector was used.