CONCLUSIONS
Sustainable and greener lactic acid production is possible using lignocellulose biomass as a raw material. In this work, we demonstrated the rapid fermentation of lactic acid from hydrolysate of sugarcane bagasse without the use of a neutralizing agent. This process was catalyzed by an originally robust microorganism engineered through only three metabolic modifications—introduction of exogenous L-LDHand double disruptions of PDC s—with retaining high tolerance to acid and inhibitor stresses. Compared with other approaches that have attempted to construct a recombinant host with extensive genetic interruptions, this strategy is efficient, more practical and expected to provide a more stable system for industrial lactic acid bioprocess that utilizes lignocellulose biomass as its feedstock.