Breeding yeast
All plasmids were digested by the restriction enzymes (NEB) shown inFigure 1 to yield the five derived strains mentioned inTable 1 . Digested plasmids were transformed to a yeast strain by following a LiAc/single-stranded carrier DNA/PEG method (Gietz & Woods, 2002). Each transformant was selected on a YPD agar containing the respective selection marker(s). Sequencing analysis using Applied Biosystems 3500 Genetic Analyzer was performed to confirm that the fragment(s) of interest were successfully inserted.