CONCLUSIONS
Sustainable and greener lactic acid production is possible using
lignocellulose biomass as a raw material. In this work, we demonstrated
the rapid fermentation of lactic acid from hydrolysate of sugarcane
bagasse without the use of a neutralizing agent. This process was
catalyzed by an originally robust microorganism engineered through only
three metabolic modifications—introduction of exogenous L-LDHand double disruptions of PDC s—with retaining high tolerance to
acid and inhibitor stresses. Compared with other approaches that have
attempted to construct a recombinant host with extensive genetic
interruptions, this strategy is efficient, more practical and expected
to provide a more stable system for industrial lactic acid bioprocess
that utilizes lignocellulose biomass as its feedstock.