Immunofluorescence
Freshly labial tissue was embedded in O.C.T. (Tissue-Tek) and 8μm frozen
sections were prepared. Sections were immersed in 95% ethanol for 15
min, blocked in normal goat serum for 3h at room temperature, followed
by incubating with 25 μg/ml mouse anti-human BDCA-2 (CD303) monoclonal
antibody (MAB62991, R&D), 1:50 diluted mouse anti-human IFN-α
monoclonal antibody (sc-373757, SANTA CRUZ BIOTECHNOLOGY, INC.) or 1:500
diluted rabbit anti-human EpCAM monoclonal antibody (also known as
CD326, ab223582, abcam) at 4°C overnight. CoraLite594-conjugated goat
anti-mouse secondary antibody (proteintech, China) or
CoraLite488-conjugated goat anti-rabbit secondary antibody (proteintech,
China) was incubated at a dilution of 1:500 for 1h at room temperature.
Further, the slides were mounted by an antifading mounting medium with
DAPI (S2110, Solarbio) and scanned by Pannoramic MIDI (3DHISTECH,
Hungary).