Fig 5. pSS plasma, IgG and IgA down-regulate the viability of pDCs. ­
a. Representative flow cytometric graph and summary graph of pDCs viability. pDCs were stimulated by HC or pSS derived plasma (n=5) for 4 hours with 100ng/ml IL3. Representative flow cytometric graph of apoptosis detection, pDCs were stimulated by increasing concentration of IgG (b) or IgA (c) for 2 hours with 100ng/ml IL3 (n=4). Statistical graph of pDCs viability when stimulated by different concentrations of human IgG (d) and IgA (e) . Unpaired two-tailed Student’s t-test was performed. *P-value < 0.05, **P-value < 0.01, ***P-value <0.001, **** P-value <0.0001.