Flow cytometry
Cells were harvested and resuspended at the concentration of 5×106 cells/ml. Fluorochrome-conjugated monoclonal antibodies for cell surface staining were as follows: anti-human CD303, CD123, Lineage, HLA-DR, CD80, CD83, CD86, CD19, CD40, CD74, CD27, IgD, CCR2, CCR4, CCR5, CCR7, CCR10, CD38, CD138, CD24.
The Intracellular Fixation & Permeabilization Buffer Set (eBioscience) was used for intracellular staining following manufacturer’s protocol. Fluorochrome-conjugated monoclonal antibodies for intracellular staining are as follows: anti-human Ki-67, TLR7, TLR9, IFN-α, TNF-α, IL-6.
Detailed information about conjugated fluorescence and manufacturers for antibodies is shown in Supplementary Table 1.