Apoptosis assay
Cell apoptosis was detected by PE Annexin V Apoptosis Detection Kit I
(559763, BD Pharmingen). Briefly, cells were washed with 1ml 1× Annexin
V binding buffer after regular surface staining, then incubated with
PE-conjugated Annexin V and 7-AAD for 15 minutes at room temperature,
all the samples were analyzed by
BD Accuri C6 flow cytometer
(Becton Dickinson, USA).