Fig 4. Co-culture of unstimulated pDCs and B cells. Purified CD19+ B cells from allogeneic healthy donors cultured alone or with pDCs (without extra activation) from pSS and HC in the presence of 100ng/ml IL3 for 7 days, the ratio of pDC to B cell was 1:10. a:Representative flow cytometric graph of ki-67 in B cells cultured alone or cultured with pDCs. b: The effect of pDCs on B cell proliferation (n=12) and the effect comparison between HC (n=6) and pSS (n=6) derived pDC on B cells.c: Expression of activation markers on B cells cultured alone or cultured with pDCs.d: Representative flow cytometric graph of B cells differentiation into plasma cells/plasmablasts. e: The effect of pDCs on B cell differentiation (n=12) and the effects comparison between HC (n=6) and pSS (n=6) derived pDCs on B cell differentiation.f: Secretion of IgG, IgA and IgM by B cells cultured alone or cultured with pDCs (n=12). Data are shown as mean ± SEM. Paired and unpaired two-tailed Student’s t-test was performed. *P-value < 0.05, **P-value < 0.01, ***P-value <0.001, **** P-value <0.0001.