2.6 | Fixation index analysis
Fixation index (Fst) was calculated using VCFTOOLS (Danecek et al., 2011) with a sliding window of 100 kb and step size of 10 kb (-window-pi 100000 -window-pi-step 10000). By using the Fst for correlation analysis to analyze the altitude distance and geographic distance between materials. Pearson Correlation Coefficient (PCC) (Eggers et al., 2003) is used to represent the phase between altitude distance and geographic distance. By using R 3.6.3 software for calculating the correlation coefficient (r) and significance level (p). The magnitude of the PCC is determined by the value of r. It reflects the degree of linear correlation between two variables, with a range of r values ranging from -1 to +1. Close to -1 indicates negative correlation, and close to +1 indicates positive correlation.