3.2 Differentially expressed genes
We compared gene expression between current-day and elevated
CO2 conditions for the CNS and eyes separately. There
was more variance in the eyes than the CNS (Figure 2A). In the CNS, we
identified 25 differentially expressed genes (DEGs) between current-day
and elevated CO2 conditions; 14 upregulated and 11
downregulated with elevated CO2. In the eyes, there were
eight DEGs; five upregulated and three downregulated at elevated,
compared to current-day, CO2 conditions (Figure 2B). Two
genes were significantly upregulated with elevated CO2 in both the CNS and eyes; one essential for autophagy (ykt6) and
another poorly characterised gene (fam204a). In both tissues, the
DEGs play roles in neurotransmission (CNS: folh1, syvn1-b, slc2a13, celsr3, eyes: maoa, slc18a1, cbs), immune function (CNS: psenen, syvn1-b,
map4k5, tf, nme6, map1l3ca/b, eyes: pglyrp2, cbs, maoa), the oxidative stress response (CNS: tf, cyb561d2, syvn1-b, chrac1, ykt6, eyes: cbs, ykt6),
and transcription regulation (CNS: nme6, chrac1, znf271, eyes: gtf2e2 ) (Table S6).