3.1 Transcriptome assembly and annotation
The de novo transcriptome assembly for Idiosepius pygmaeus was created from a total of 138.6 million PacBio ISO-sequencing subreads
and resulted in 49,981 transcripts that were clustered into 27,420
genes. The transcriptome assembly had an N50 of 3,163 bp, 70.4%
complete BUSCOs and an 82.1 ± 5.5% overall alignment rate of the
RNA-seq reads (Table S3). A total of 69% of the transcripts received a
functional annotation (Supplementary Table S4). The species distribution
of the top blast hits was dominated by cephalopod species (Figure S22).
Final mapping of RNA-seq reads against the transcriptome assembly had a
73.6 ± 6.9% mapping rate (Table S5).