2.1 Specimen collection and library preparation
Species sequenced in this study included five species within themacrotis group and one closely related species R. pusillus(Table 1). Samples of the brain, liver, and cochlear were biopsied quickly after euthanasia, placed in RNA later, and then stored at -80℃ until RNA extraction. To avoid the influence of sex, we chose mature females and ensure none were pregnant. Total RNA from these tissues was extracted using TRIzol reagent (Invitrogen Corp., Carlsbad, CA). RNA purification was performed using an RNeasy Mini Kit (Qiagen, Chatsworth, CA). Library constructions from the brain, liver, and cochlear of each species according to the Illumina Hiseq 2500 RNA sample preparation kit (Illumina, San Diego, CA). We constructed two paired-end libraries with insert sizes of 150 base pairs (bp). All these original data have been deposited into the NCBI Sequence Read Archive database (Accession Number: SAMN35883750-SAMN35883755) (Supporting Information Table S1).