PRS Construction
We separated the 70% sample size of our cohort to construct the
training GWAS summary statistics. PRS-CS24 was used to
infer the posterior effect size of SNPs using the IgE summary statistics
of the training group. The 1000 genome EAS phase3 samples were used as
LD reference panel. We further adopted the effect size in the rest of
30% samples and used PLINK2.025 to sum up the effect
allele dosages of each individual and generate the IgE PRS. We checked
the correlation between IgE PRS and IgE using Pearson
correlation coefficient test. In order to investigate the association
between different quantiles of IgE PRS groups and IgE, we divided the
IgE PRS into 11 parts according to the IgE PRS quantiles (0-10th,
10-20th, 20-30th, 30-40th, 40-50th, 50-55th, 55-60th, 60-70th, 70-8th,
80-90th, and 90-100th). The population within the 50th to 55th quantile
range was selected as the reference group. The logistic regression was
performed compared with each group with the reference group and adjust
with age, sex, and PC1 to PC10.