Subcellular location of BnGSTU12
The CDS of BnGSTU12 was inserted into pEGAD vector containing the eGFP tag and CaMV 35S promoter with EcoRI and BamHI restriction site.Agrobacterium strain GV3101 containing recombinant plasmid injected into tobacco leaves. The fluorescence was observed after 2-3days by confocal fluorescence microscope.
Histochemical GUS Analysis
The promoter (upstream 2000 bp of ATG) of BnGSTU12 was cloned into pCAMBIA1391 vector with SalI and EcoRI restriction site. The recombinant vector was transferred to A. thaliana by Agrobacterium-mediated floral transformation. Different tissues of positive plants were collected and stained with GUS solution. After decolorization with ethanol, GUS-stained tissues were observed under the microscope.