JA played the great role in BnGSTU12 mediated S.
sclerotiorum resistance
To
investigate
whether the JA and SA pathway was involved
inGSTU12 mediated S. sclerotiorum resistance, we measured
the JA and SA content in WT and overexpressed A. thaliana .
Compared to WT (1.21 ng/g), JA content (1.40 ng/g) was higher in
overexpressing lines (Figure 8A). JA content increased in WT and
BnGSTU12-OE lines after 6 h inoculation withS.
sclerotiorum , but
JA
content (3.32 ng/g, 2.37-fold) increased more in
theBnGSTU12 -OE than WT plants (1.68 ng/g, 1.39-fold)
(Figure
8A). However, the SA content decreased in BnGSTU12 -OE lines after
inoculation withS.
sclerotiorum (Figure 8B). In addition, the expression of genes involved
in JA and SA pathway were investigated (Figure 8C and Supplementary
Figure 3). The expression level of JAR gene involving in JA-Ile
biosynthesis, and JA-responsive genes (PDF1.2 ) were higher in OE
lines than WT after 12 and 24 h inoculation with S. sclerotiorum(Figure 8C). The expression level of JAZ gene respressing JA
signaling was less in OE lines than
WT. The genes involved in SA signlaing pathway and SA-responsive
(NPR1 , PR1 and PR3 ) induced less in overxpressingA. thaliana lines than WT after inoculation with S.
sclerotiorum (Supplementary Figure 3). The results showed that JA
pathway
played the great role in BnGSTU12 mediated S. sclerotiorumresistance.