Subcellular location of BnGSTU12
The CDS of BnGSTU12 was inserted
into pEGAD vector containing the
eGFP tag and CaMV 35S promoter with EcoRI and BamHI restriction site.Agrobacterium strain GV3101
containing recombinant plasmid injected into tobacco leaves. The
fluorescence was observed after 2-3days by confocal fluorescence
microscope.
Histochemical GUS Analysis
The promoter (upstream 2000 bp of ATG) of BnGSTU12 was cloned
into pCAMBIA1391 vector with SalI and EcoRI restriction site.
The recombinant vector was
transferred to A. thaliana by Agrobacterium-mediated floral
transformation. Different tissues of
positive plants were collected and stained with GUS solution. After
decolorization with ethanol, GUS-stained tissues were observed under the
microscope.