Abiotic and biotic stress treatment
B. napus at four-leaf stage were used for abiotic and biotic
stress treatment. The leaves ofB.
napus were obtained at 0, 3, 9, 24, 48, 72 h after treated with 10%
PEG 6000 and 0.9% NaCl to simulate drought and salt stress. After
ultraviolet (UV) stress, B. napus leaves were obtained at 0, 20,
40, 60, 180 and 540 minutes. The leaves were sprayed with different
hormones, 1.5 mg/L KT, 10 μM ABA, 1 mM SA, 100 μM GA3, 100 μM MeJA,
0.04% ETH, and sampled at 0, 1, 3, 9, 24 and 48 h. For S.
sclerotiorum infection, theS. sclerotiorum was cultured
on PDA plate (20% potato, 2% dextrose, and 1.5% agar). TheSclerotinia mycelium agar plug were placed on B. napusleaves, and the B. napus leaves were harvested 0, 6, 12, 24, 48
and 60 h post inoculation. For all the stress treatment, each sample was
collected from five plants. All collected samples were rapidly frozen in
liquid nitrogen for RNA extraction and gene expression analysis.