Genetic transformation of A. thaliana and B.
napus
The full-length coding sequences of BnGSTU12 was cloned into
plant binary expression vector
pL-DsRed. The recombinant vector was
introduced into Agrobacterium GV3101. A. thalianatransformation was performed by the floral dipping method (Clough and
Bent 1998). The B. napus line ZS11 was used as the receptor and
transformed using the Agrobacterium-mediated transformation protocol
(Yin et al. 2022).
Agrobacterium-mediated transient expression inN. benthamiana leaves
and trypan blue staining
The empty vector (EV) as the control
and 35S:BnGSTU12-DsRed were transformed into tobacco leaves. Three days
after infiltration,N.
benthamiana leaves were infected with S. sclerotiorum and
lesion area was measured 36 h after
inoculation. N. benthamianaleaves was stained by trypan blue. The infected tobacco leaves were
decolorized with ethanol, and then
submerged to trypan blue solution (0.1% trypan into lactic acid:
glycerol: water (1: 1: 1) solution) for 15min. N. benthamianaleaves were cleaned with water and photographed.