Expression pattern analysis of BnGSTU12
The expression patterns of BnGSTU12 were identified in different
tissues in B. napus through qRT-PCR. BnGSTU12 was
expressed in all tissues, and highly expressed in
roots,
stem, leaves,
stamen
and mature seeds (Figure 2). To complement the expression by qRT-PCR
above, we investigated the spatio-temporal expression patterns ofBnGSTU12 in transgenic A. thaliana by fusing the 1.5 kb
promoter region of BnGSTU12 to the β-GLUCURONIDASE(GUS ) reporter gene. BnGSTU12 was predominantly expressed
in roots, stems and leaves in the seedling at 10 days and 30 days
(Supplementary Figure 1A, B, C). During the flowering stage, higher
expression of BnGSTU12 was observed in sepals, stamens and
pistils (Supplementary Figure 1D).