JA played the great role in BnGSTU12 mediated S. sclerotiorum resistance
To investigate whether the JA and SA pathway was involved inGSTU12 mediated S. sclerotiorum resistance, we measured the JA and SA content in WT and overexpressed A. thaliana . Compared to WT (1.21 ng/g), JA content (1.40 ng/g) was higher in overexpressing lines (Figure 8A). JA content increased in WT and BnGSTU12-OE lines after 6 h inoculation withS. sclerotiorum , but JA content (3.32 ng/g, 2.37-fold) increased more in theBnGSTU12 -OE than WT plants (1.68 ng/g, 1.39-fold) (Figure 8A). However, the SA content decreased in BnGSTU12 -OE lines after inoculation withS. sclerotiorum (Figure 8B). In addition, the expression of genes involved in JA and SA pathway were investigated (Figure 8C and Supplementary Figure 3). The expression level of JAR gene involving in JA-Ile biosynthesis, and JA-responsive genes (PDF1.2 ) were higher in OE lines than WT after 12 and 24 h inoculation with S. sclerotiorum(Figure 8C). The expression level of JAZ gene respressing JA signaling was less in OE lines than WT. The genes involved in SA signlaing pathway and SA-responsive (NPR1 , PR1 and PR3 ) induced less in overxpressingA. thaliana lines than WT after inoculation with S. sclerotiorum (Supplementary Figure 3). The results showed that JA pathway played the great role in BnGSTU12 mediated S. sclerotiorumresistance.