Expression pattern analysis of BnGSTU12
The expression patterns of BnGSTU12 were identified in different tissues in B. napus through qRT-PCR. BnGSTU12 was expressed in all tissues, and highly expressed in roots, stem, leaves, stamen and mature seeds (Figure 2). To complement the expression by qRT-PCR above, we investigated the spatio-temporal expression patterns ofBnGSTU12 in transgenic A. thaliana by fusing the 1.5 kb promoter region of BnGSTU12 to the β-GLUCURONIDASE(GUS ) reporter gene. BnGSTU12 was predominantly expressed in roots, stems and leaves in the seedling at 10 days and 30 days (Supplementary Figure 1A, B, C). During the flowering stage, higher expression of BnGSTU12 was observed in sepals, stamens and pistils (Supplementary Figure 1D).