Figure 2. Validation of D-tagatose synthetic
pathways. All reactions were performed with 20 mM lactose, 100 mM
Tris-HCl (pH 8.0) at 30 °C. The information on pathway conditions is
shown in Table 3. The yields of tagatose and galactitol in (A) Pathway
I–Condition 1, (B) Pathway I–Condition 2, and (C) Pathway
II–Condition 1 are shown. Experiments were performed in triplicates,
and error bars represent the standard deviation.