2.7 Statistical analysis
Data are expressed as mean and standard deviation of the mean (mean ± SD). For statistical analysis, the normality of the variables was analyzed using the Bartlett test. For non-parametric variables, Kruskal-Wallis was used followed by Dunn’s post hoc. For parametric variables, one-way variance (ANOVA) by Tukey’s post hoc was used, and for specific comparisons between groups, the student t-test was used.
For the evaluation of the mothers, food consumption and body weight, Kruskal-Wallis followed by Dunn’s post hoc were used. Fasting blood glucose, insulin tolerance test, and the comet assay were analyzed by one-way ANOVA with Tukey’s post hoc. Differences in the fructose serum levels and micronucleus test were tested with the student t-test.
For the evaluation of the offspring (males and females), food consumption, fructose serum levels, fasting glucose, insulin tolerance test, adiposity index and comet assay, ANOVA with Tukey’s post hoc was used. Micronucleus test were analyzed with the student t-test. Body weight and histological parameters were tested by Kruskal-Wallis followed by Dunn’s post hoc. Differences in micronuclei was tested by student t-test.
Differences between groups were considered significant when P values were less than 0.05 (p<0.05), using the GraphPad Prism 5.0 program.