2.2.2 Voluntary Physical Exercise (VPE)
Voluntary physical exercise was performed using a voluntary running wheel placed inside the cages during the treatment time (varying according to each group), thus allowing free access by the animals. The exercise mileage of the animals was controlled through an infrared equipment placed next to the wheel and was divided by the animals present in the cage (5 animals) (Muller et al., 2011). This analysis was performed in the pre-pregnancy period (8th week) and during pregnancy (12th week). The females during the pre-pregnancy period ran an average of 3143 meters/day and in the gestation period 5649m/day, and this amount traveled was an estimate per animal.