2.2.1 Fructose
Mothers received drinking water or fructose at a concentration of 20%/L
ad libitum in hydration bottles during pre-pregnancy (8 weeks of
treatment), copulation (7 days), pregnancy (21 days) and lactation (21
days) periods. These dosages and treatment time were chosen according to
data previously described in the literature
(Mamikutty et al., 2014; Magenis
et al., 2020). The route of administration and the period of treatment
were chosen in order to maintain the relevance of the applicability of
human studies. Fructose (Sigma Aldrich) was dissolved in drinking water
and prepared three times a week at a concentration of 20%/L (Magenis et
al., 2020).