Recording Solutions
In all experiments, the extracellular solution contained (in mM): NaCl
140, CaCl2 2, MgCl2 0 to 2, KCl 3, HEPES
10, D-glucose 10 (pH = 7.4, 330 mOsm). For neurons, Glycine 0.01,
bicuculline 0.01, and tetrodotoxin 0.0003 were added the day of the
experiment. For whole-cell voltage clamp recordings, the pipette was
filled with an intracellular solution (in mM): CsCl 140, EGTA 5,
CaCl2 0.5, HEPES 10, ATP-Na2 2 and
D-glucose 10 (pH = 7.2, 300 mOsm). Equimolar solutions containing
increased KCl concentrations (from 3mM to 100 mM) were obtained by equal
decrease of NaCl concentration.