Quantitative Real-time Polymerase Chain Reaction (qPCR)
The expression of mRNA encoding for indicated genes in THP-1cells or
BMDMs was quantified by qPCR with the SYBR® Premix Ex Taq™ (#RR820A,
Takara). Table 1 showed the main primers sequence used in this
experiment. The relative expression of target gene was calculated by the
2−ΔΔC t method.
Table 1 Primer sequences