Cell viability
Assess the viability of cells by using the CCK-8 assay. Briefly, THP-1
cells or BMDMs were plated overnight in 96-well plates at a cell density
of 1x105 cells/well. Cells were treated with various
concentrations of AB4, AA3, and 23-HA (50, 100, 200µM) for 24 h, with
Nacl or 0.1% DMSO as control. All samples were then incubated with 90
µl fresh medium and 10 µl CCK-8 at 37°C for 2h. Absorbance was measured
with a microplate reader at 450 nm. Experiments were performed
independently three times.