Figure 3. AB4 specifically inhibits NLRP3 inflammasome activation in colonic macrophages. (A ) Production of IL-1β, IL-18, IL-6, iNOS, and TNF-α in the serum of DSS-induced colitis mice was determined by ELISA. (B ) The indicated proteins of colonic homogenate-related factors were determined by Western Blot. (C ) The mRNA levels of colonic homogenate-related factors were determined by qPCR. Western Blot was used to detect NLRP3, Caspase-1, and IL-18 protein expression in colon macrophages (D ) and epithelial cells (E ), and ELISA was used to detect IL-1β protein expression. Data are presented as mean ± SD. **P < 0.01vs. Normal group; #P < 0.05,##P < 0.01 vs. DSS group.