FITC-dextran intestine-blood barrier tests
To analyze the permeability of the epithelial barrier in mice, on the
7th day, mice in each group were deprived of water and fasted for 4 h,
and then each mouse was gavaged with
FITC-dextran (0.6 mg/g; Sigma-Aldrich). The blood was collected after 4
h, and the content of FITC-dextran in serum was measured with a
fluorescence spectrophotometer setup with an emission wavelength of 490
nm and an excitation wavelength of 520 nm.