Methods
Chemicals and reagents
AB4 (C59H96O26, MW: 1221.38, >98% purity), AA3 (C41H66O12, MW: 750.96, >98% purity), and 23-HA (C30H48O4, MW: 472.7, >98% purity) were purchased from Sichuan weikeqi-biotech (Chengdu, China). 5-ASA was purchased from Ipsen Pharma (Houdan, France). Dulbecco’s modified Eagle medium (DMEM), RPMI-1640, Fetal bovine serum (FBS), and penicillin-streptomycin were purchased from Gibco (Grand Island, USA). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). Lipopolysaccharides (LPS), adenosine triphosphate (ATP), phorbol myristate acetate (PMA), and Nigericin (Nig) were purchased from Sigma-Aldrich (St. Louis, USA). Dextran sulfate sodium (DSS, molecular weight 36-50kDa) was purchased from MP Biomedicals (Solon, USA). Recombinant Murine M-CSF was purchased from PEPROTECH (Rocky Hill, USA).