Figure 5. AB4 inhibits the activation of NLRP3 inflammasome in
vitro. (A-B ) BMDMs were pretreated with AB4 (50, 100, and
200μM) for 4h, and then cultured with LPS (1μg/ml) for 6h. The mRNA
expression of related components in NLRP3 inflammasome was analyzed by
qPCR (A ). The protein expression of related components in NLRP3
inflammasome was analyzed by Western Blot (B ). Data are
presented as mean ± SD. **P < 0.01 vs. Control group;#P < 0.05, ##P
< 0.01 vs. LPS group. (C-E ) BMDMs and
differentiated THP-1 cells were pretreated with AB4 (50, 100, and 200μM)
for 4h, and then cultured with LPS(1μg/ml) for 6h, followed by
incubation with ATP (5 mM) for 30min, nigericin (10 μM) for 30min. The
protein expressions of NLRP3, Caspase-1, IL-1β, and IL-18 were
determined by Western Blot in BMDMs (C ) and THP-1 cells
(D ); the production of IL-1β and IL-18 was analyzed by ELISA
(E ). Data are presented as mean ± SD. **P <0.01 vs. Control group; #P< 0.05 , ##P <0.01 vs. LPS+ATP or LPS+Nig group.