Figure 5- Impact of the four breeding regimes, simulated over 50 generations. Showing the impact on (A) the genetic load, (B) the realised load of offspring, (C) the fitness of adults, and (D) neutral nucleotide diversity (π). Each coloured line corresponds to a specific mating regime: ”Random mating” (grey), ”Minimise relatedness” (blue), ”Minimise load” (orange), and ”Minimise load and relatedness” (green). The genetic load and realised load are expressed in lethal equivalents calculated using equations [1] and [2] in the Material & Methods (see ). The values presented in the figure represent the mean results obtained from 100 replicas.