Cell line and Cell culture
PLC/PRF/5 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (Gibco, Life Technologies, Carlsbad, CA, USA) in a humidified incubator maintained at 37°C and 5% CO2.