Figure Legends
Fig. 1. The effects of the Montmorillonite (Mt) and Palygorskite (Pal) on (A) the maximum quantum yields of photosystem II reaction center and (B) electron transport rate. The vertical bar represents the standard deviation of triplicate samples.
Fig. 2. A: P uptake of Chlamydomonas reinhardtii cultured at 3.15 mg/L and 31.5 mg/L P concentrations. B: The number of counts (per 107 cells) of C. reinhardtii after four days incubation. *P<0.05. C: CO2 emission (per 1010 cells) ofC. reinhardtii . The error bars represent the standard deviation based on three parallel experiments. *P<0.05; NS, not significant.
Fig. 3. Three-dimensional excitation-emission matrix fluorescence spectra of the supernatant under different treatments. A: the four fluorescent components (C1-C4) identified by PARAFAC analysis; B: relative abundances of fluorescent components under different treatments.
Fig. 4. The content of proteins (A), polysaccharides (B), and photosynthetic pigments (C) after four days of incubation.
Fig. 5. ATR-IR spectra of C. reinhardtii cells and two minerals. 200Mt, 500Mt, 200Pal, and 500Pal: spectra of the algal cells incubated with 200, 500 mg/L Mt/Pal. Mt and Pal: spectra of two minerals. 0: spectra of the algal cells.
Fig. 6. A and D: SEM images of Mt (A) and Pal (D). B, C, E, and F: SEM imaging on the algae cells under 200 (B&E), 500 (C&F) Mt/Pal.
Fig. 7. TEM images of C. reinhardtii cells incubated with 200 (A), 500 (B) mg/L Mt and 200 (C), 500 (D) mg/L Pal of the medium in all the treatments.
Fig. 8. Main metabolic pathway network related to differentially expressed proteins (DEPs). The color bar indicates the up-down adjustment of DEPs (C for CK; P for Pal (200 mg/L); M for Mt (200 mg/L)). IF2CP, CPLS1: genes related to the transformation of precursor substances into chloroplasts; CAB4, CAB7 and CHLG: genes related to chlorophyll synthesis; SAMC1 and odhA: genes related to cellular respiration; psbH and OHP1: genes related to PhotosystemII synthesis.