Determination
of seed kernel oil content and fatty acid composition
Each plant’s seeds weighed about 10g, and calibration samples of pureA. truncatum oil with known oil content were taken. The oil
content of the calibration samples was quickly determined by utilizing
the SPEC-PMR pulsed nuclear magnetic resonance instrument oil and water
analyzer, and each copy was repeated three times[16,
17].
The fatty acid fraction of A. truncatum seed kernel was
determined based on the external standard method specified in GB
5009.168-2016. After methyl esterification of A. truncatum seed
oil samples in sulfuric acid medium, the supernatant was obtained, and
the fatty acid standard assay solution and the sample to be measured
were separately determined on the machine, and then the chromatograms
were obtained by GC analysis. GC/MS conditions: DA-2560 capillary column
(100 m × Ф0.25 mm × 0.2 μm); high purity nitrogen carrier gas; inlet
temperature of the injector is 270 ℃, the Detector temperature was 280
℃; the initial column temperature was 100 ℃ (lasted 13 min),
100~180 ℃ (heating rate 10 ℃/min, lasted 6 min),
180~200 ℃ (heating rate 1 ℃/min, lasted 20 min),
200~230 ℃ (heating rate 4 ℃/min, lasted 10.5 min). The
fatty acid content was obtained by peak area
normalization[18-20], and the composition obtained
from the determination is shown in Table
2.
Table 2 Fatty acid composition of samples