3.2 | Phylogenetic relationships
For samples of 2016, the network separated the 10 ribotypes of the
nuclear ITS into three different monophyletic lineages. Clearly, the
ribotypes R1, R2, R6, and R10 that are present in the northwest (NW)
group of populations formed a clade; R5 and R7–R9 dominating the south
(S) and southeast (SE) populations had the closest phylogenetic
relationship to the NW clade, while the R3 and R4 ribotypes that are
exclusive to the southwest (SW) populations formed an independent clade
from the NW one (Figure 2a). Similarly, for the 2022 samples, the
network separated the 18 ribotypes of the nuclear ITS into three
monophyletic lineages. The ribotypes R1, R2, R11, R15–R17, and R18,
which are dominating the NW populations, converged into a group, R7–R10
and R12–R14 from the S and SE groups showed the closest phylogenetic
relationship, and R3–R5, and R6, which are exclusive to the SW group,
formed an independent clade (Figure 2c). In addition, BI and ML trees
with high PP/BS values based on the ribotypes showed very similar
results (Figures 2a, 2c).
Regarding the cpDNA, the pattern of phylogenetic relationships was very
close to that of nrDNA. For both sampling periods (2016 and 2022), the
network of chlorotypes showed three monophyletic lineages (Figures 2b,
2d). The chlorotypes most abundant in the NW group of populations
(C1–C8 and C16 in 2016; C1–C6, C13, C14, and C15 in 2022) formed a
clade, which was closest to the clade formed by the chlorotypes from the
S and SE group of populations (C12–C15 in 2016, C9–C12 in 2022); the
clade formed by the chlorotypes exclusive to the SW group (population
JF; C9 and C10 in 2016, C7 and C8 in 2022; Figures 2b, 2d) was the most
distant with respect to the NW one. Again, BI and ML trees with high
BS/PP values based on chlorotypes showed very similar results (Figures
2b, 2d).