For the 2022 samples, the ITS1/2 sequence matrix comprised 640 bp in
total (Table 2). Twenty-six S were present, which allowed the
identification of 18 different ribotypes from a size of 117 samples
(Table 2). At the species level, π was 0.00464, Nd was
2.912, and h was 0.876 (Table 2). Two ribotypes, R1 and R11, were
shared by at least two populations (R1 was present in BW, YJ, and EX,
and ribotype R11 occurred in YG and WZ). The other 16 ribotypes were
private (Table 3; Figure 1b). Comparing samples between 2016 and 2022,
there was a decrease in both the number (three in 2016 to two in 2022)
and the percentage of shared ribotypes (30.0% in 2016 and 11.1% in
2022) (Table 3). Notably, the sharing of ribotypes among the three
groups of populations separated by the expressway network (NW, S, and
SE) is much diminished; while in 2016 R1 and R6 were shared by NW and
SE, and R6 shared by NW, S, and SE, in 2022 only R11 was shared by NW
and SE (Figure 1b).