Figure 1 Haplotype distribution of (a ) nrDNA ITS and
(b ) cpDNA trn L-F and ycf 1b in the years of 2016
and 2022 of eight populations of Primulina heterotricha on Hainan
Island, south China.
The total length of the combined chloroplast alignments in 2016 was 1405
bp (730 bp and 674 bp in size for trn L-trn F andycf 1b, respectively). The alignment contained 48 S , and 16
chlorotypes (Nn ) were present among the 176 samples (Table 2). At
the species level, π was 0.00940, Nd was 12.725, andh was 0.907 (Table 2). Of the 16 chlorotypes, only two (C1 and
C2) were shared between populations (BW and YJ). The other 14
chlorotypes were private (Table 3; Figure 1c). For the 2022 samples, the
combined alignment of two cpDNA regions comprised 1592 bp (870 bp and
722 bp in size for trn L-trn F and ycf 1b,
respectively) which contained 42 S , and 15 Nn were present
among the 117 samples (Table 2). At the species level, π was
0.00868, Nd was 13.637, and h was 0.874 (Table 2). Of the
15 chlorotypes, only two (C1 and C3) were shared (C1 was shared by BW,
YJ, and EX, and C3 by BW and YJ) (Table 3). The other 13 chlorotypes
were private (Table 3; Figure 1d). In
sum, and contrary to the ITS, there were no major differences between
the 2016 and 2022 cpDNA datasets, with the same number (two) and almost
the same percentage (12.5% vs. 13.3%) of shared chlorotypes, all
occurring on the NW group of populations.