Table 2 Primer polymorphic sites (S), number of ribotypes/chlorotypes (Nn), haplotypes diversity (h), nucleotide diversity (π), the average number of nucleotide difference (Nd) for nrDNA and cpDNA amplification and genetic diversity in 2016 and 2022 in Primulina heterotricha. Table 2 Primer polymorphic sites (S), number of ribotypes/chlorotypes (Nn), haplotypes diversity (h), nucleotide diversity (π), the average number of nucleotide difference (Nd) for nrDNA and cpDNA amplification and genetic diversity in 2016 and 2022 in Primulina heterotricha. Table 2 Primer polymorphic sites (S), number of ribotypes/chlorotypes (Nn), haplotypes diversity (h), nucleotide diversity (π), the average number of nucleotide difference (Nd) for nrDNA and cpDNA amplification and genetic diversity in 2016 and 2022 in Primulina heterotricha. Table 2 Primer polymorphic sites (S), number of ribotypes/chlorotypes (Nn), haplotypes diversity (h), nucleotide diversity (π), the average number of nucleotide difference (Nd) for nrDNA and cpDNA amplification and genetic diversity in 2016 and 2022 in Primulina heterotricha. Table 2 Primer polymorphic sites (S), number of ribotypes/chlorotypes (Nn), haplotypes diversity (h), nucleotide diversity (π), the average number of nucleotide difference (Nd) for nrDNA and cpDNA amplification and genetic diversity in 2016 and 2022 in Primulina heterotricha. Table 2 Primer polymorphic sites (S), number of ribotypes/chlorotypes (Nn), haplotypes diversity (h), nucleotide diversity (π), the average number of nucleotide difference (Nd) for nrDNA and cpDNA amplification and genetic diversity in 2016 and 2022 in Primulina heterotricha.
DNA fragment ITS ITS trnL-F and ycf1b trnL-F and ycf1b trnL-F and ycf1b trnL-F and ycf1b
2016 2022 2022 2016 2022 2022
S 13 26 26 48 42 42
Nn 10 18 18 16 15 15
h 0.809 0.876 0.876 0.907 0.874 0.874
π 0.00435 0.00464 0.00464 0.00940 0.00868 0.00868
Nd 2.98812 2.91217 2.91217 12.72468 13.63660 13.63660
Fragment size 701 640 640 1405 1592 1592
Tajimas’ D 0.80474 -1.18345 -1.18345 1.38922 2.25892 2.25892
Fu’s Fs 1.811 -3.937 -3.937 12.439 11.3 11.3