2.2 Experimental grouping
To observe the effect of NSC-Exos treatment on TLR4/NF-κB signaling
pathway, rats were randomly divided into Sham group, IRI group, PBS
group, inhibitor TAK-242 group, and NSC-Exos transplantation group
(n=20). The neuroprotective effect of NSC-Exos was assessed by measuring
cerebral water volume, infarct volume, and expression of inflammatory
factors. The expression of TLR4 and NF-κB was detected by qPCR and
western blot to explore the protective mechanism of microglia-mediated
inflammation.