3.7 NSC-Exos regulates the polarization of microglia
To further investigate the polarization status of microglia after
injury, immunofluorescence staining was used to evaluate the
colocalization expression of M1 (CD86/ Iba1) and M2 (CD206/ Iba1). The
CD86/ Iba1 staining results of M1 microglia showed that
CD86+/Iba1+ colocalization
expression was significantly increased in IRI and PBS groups compared
with Sham group (P< 0.01). Compared with IRI group and
PBS group, CD86+/Iba1+colocalization expression was significantly decreased in NSC-Exos group
(P< 0.01, Fig. 7a, c). The CD206/Iba1 staining results
of M2 microglia showed that compared with Sham group,
CD206+/Iba1+ colocalization
expression was increased in IRI and PBS groups
(P< 0.01). Compared with IRI group and PBS group, the
expression of CD206+/Iba1+colocalization was significantly increased in NSC-Exos group
(P< 0.01, Fig. 7b, d).