2.9 Immunohistochemical staining
The expression of the microglial marker Iba1 in the cerebral cortex was
observed. Paraffin sections of brain tissue were routinely dewaxed to
water, peroxidase inhibited by 3% H2O2(10 min), microwave antigen repair by sodium citrate (20 min), cooled to
room temperature, sealed by 5% bovine serum albumin (60 min), incubated
overnight with primary antibody. On the second day, the slices were
taken out and rewarmed (30 min), DAB color development was photographed
for observation (Beijing Solarbio Science & Technology Co., Ltd.).