2.8 TTC staining
2,3,5-triphenyl tetrazolium chloride (TTC, Beijing Solarbio Science & Technology Co., Ltd.) staining to assess cerebral infarction. After anesthesia, the rats were injected with normal saline, the brain tissue was quickly removed and placed in -20℃ quick-frozen (30 min), and 2.0% TTC staining solution was incubated in dark (25 min), 4% paraformaldehyde was fixed, and photographs were taken for observation. Image J calculates the infarct size of the cerebral hemispheres. Infarct size (%) = (area of the opposite hemisphere - area of normal brain tissue on the same side)/area of the opposite hemisphere ×100%.