3.1 NSC-Exos improved the neurological deficits and reduced the
infarct size in MCAO model rats
To investigate the neuroprotective effects of NSC-Exos, navigational
impairment and balance beam tests were performed on MCAO model rats.
Compared with Sham group, IRI group and PBS group had obvious symptoms
of nerve dysfunction, basically could not be stabilized on balance beam,
and nerve dysfunction score was significantly increased
(P< 0.01). Compared with IRI group and PBS group, the
motor function of NSC-Exos group was restored, and the balance beam was
basically grasped, and the neurological function deficit score was
decreased (P< 0.05, Fig. 1a, b). TTC staining was used
to assess the extent of infarction, in which normal tissue is red and
infarct tissue is white. Compared with Sham group, IRI group and PBS
group had obvious white infarct areas and the infarct size increased
significantly (P< 0.01). Compared with IRI group and PBS
group, NSC-Exos group showed a small number of infarcts and decreased
infarct size (P <0.01, Fig. 1c, d). There was no
significant difference between IRI group and PBS group (P>0.05).