2.8 TTC staining
2,3,5-triphenyl tetrazolium chloride (TTC, Beijing Solarbio Science &
Technology Co., Ltd.) staining to assess cerebral infarction. After
anesthesia, the rats were injected with normal saline, the brain tissue
was quickly removed and placed in -20℃ quick-frozen (30 min), and 2.0%
TTC staining solution was incubated in dark (25 min), 4%
paraformaldehyde was fixed, and photographs were taken for observation.
Image J calculates the infarct size of the cerebral hemispheres. Infarct
size (%) = (area of the opposite hemisphere - area of normal brain
tissue on the same side)/area of the opposite hemisphere ×100%.