2.10 Immunofluorescence detection
The paraffin sections were dewaxed to water by the usual procedure described above. After sealing, the primary antibody was added directly without cleaning. The primary antibodies were CD86 (cat. no. 91882; Cell Signaling; 1:100), CD206 (cat. no. 24595; Cell Signaling; 1:100) and Iba1 (cat. no. 17198; Cell Signaling; 1:100). Nuclei were labeled with DAPI from Beyotime Institute of Biotechnology, the issues were sealed with anti-fluorescence quenched plates and the images were taken with a confocal laser microscope (Olympus, Japan).