Figure 2. FAIMS enabled Glycoproteomic analysis of chimericpglOs within N. gonorrhoeae. A) Glycan specific immunoblotting supports changes in glycosylation with N. gonorrhoeae strains expressing chimeric pglOs. B) Raincloud plots of unique N. gonorrhoeae glycoproteins and glycopeptides observed within strains expressing different pglOs withpglOmeningitidis andpglOcinerea alleles denoted in red and pink.C & D) Pie chart and Upset plots of glycoproteins observed across N. gonorrhoeae expressing chimeric PglOs reveals most glycoproteins are observed in a limited number of strains with the highest number of unique glycoproteins observed withinpglOmeningitidis and only five glycoproteins identified across all strains.