Figure 1. FAIMS fractionation enables the enrichment of glycopeptides withinN. gonorrhoeae MS11. A) FAIMS fractionation reveals the enrichment of glycopeptides at high FAIMS CVs. Blue dots denote the numbers of unique glycopeptides across individual biological replicates at each FAIMS CV. B & C) Pie chart and bar plots demonstrating that high FAIMS CVs contained the highest number of unique glycopeptides identified across biological replicates with a FAIMS CV of -30 resulting in the highest number of unique glycopeptides not observed in other CVs.D) Venn diagram of FAIMS identified glycoproteins compared to previously reported N. gonorrhoeae glycoproteins [26] supports that FAIMS analysis allows the identification of multiple novel N. gonorrhoeaeglycoproteins.