Figure 4. Proteomic alterations observed across N. gonorrhoeae strains expressing different pglO alleles compared to pglOmeningitidis. A) Upset plot of proteome alterations compared to pglOmeningitidisreveal 76 protein alterations between different alleles. B)Heatmap of unsupervised clustered z-scored alterations support differentpglO alleles lead to different proteomic effects with changes in membrane proteins highlighted in blue, transporters, highlighted in red and the single glycoprotein differentially impacted (NGO_1577) highlighted in green. Unsupervised clustering of biological replicates denoted by coloring of the dendrogram branches.