Figure 3. Proteomic alterations observed across N. gonorrhoeae strains expressing different pglO alleles.A) PCA analysis of strains expressing different pglOalleles reveals clustering of biological groups and minimal separation of groups across the PC1 and PC2 dimensions. B) Pie chart of proteins observed altered across the proteome supports that >30% of proteins appear impacted within at least onepglO alleles by ANOVA analysis. C) Heatmap of unsupervised clustered Z-scored proteomic alterations supports widespread changes across biological groups revealing differentpglO alleles impact the proteome in different ways with three broad clusters of proteome alterations observed across strains. Unsupervised clustering of biological replicates denoted by coloring of the dendrogram branches.