Figure
1. FAIMS fractionation enables the enrichment of glycopeptides withinN. gonorrhoeae MS11. A) FAIMS fractionation reveals the
enrichment of glycopeptides at high FAIMS CVs. Blue dots denote the
numbers of unique glycopeptides across individual biological replicates
at each FAIMS CV. B & C) Pie chart and bar plots demonstrating
that high FAIMS CVs contained the highest number of unique glycopeptides
identified across biological replicates with a FAIMS CV of -30 resulting
in the highest number of unique glycopeptides not observed in other CVs.D) Venn diagram of FAIMS identified glycoproteins compared to
previously reported N. gonorrhoeae glycoproteins
[26] supports that FAIMS analysis
allows the identification of multiple novel N. gonorrhoeaeglycoproteins.