Figure 2. FAIMS enabled Glycoproteomic analysis of chimericpglOs within N. gonorrhoeae. A) Glycan specific
immunoblotting supports changes in glycosylation with N.
gonorrhoeae strains expressing chimeric pglOs. B) Raincloud
plots of unique N. gonorrhoeae glycoproteins and glycopeptides
observed within strains expressing different pglOs withpglOmeningitidis andpglOcinerea alleles denoted in red and pink.C & D) Pie chart and Upset plots of glycoproteins observed
across N. gonorrhoeae expressing chimeric PglOs reveals most
glycoproteins are observed in a limited number of strains with the
highest number of unique glycoproteins observed withinpglOmeningitidis and only five glycoproteins
identified across all strains.