Figure 5. Peptide-centric analysis of non-glycosylated peptides
within the N. gonorrhoeae proteome expressing differentpglO alleles. A) Pie chart of peptides which can be
glycosylated identifiable with DIA analysis. B)Glycoprotein/Peptide analysis of NGO_1365 reveals no alterations in the
protein levels yet the peptide384EWAPSENQAAAPQAGVQTASEAKPASEAK412demonstrates variations in relative abundance across all strains.C & D) Glycoprotein/Peptide analysis of NGO_1800 and
NGO_2092 reveals no alterations in protein levels yet the lack of
peptides 173VAVGVQTASGAQTVR187 and31EQAVSAAQSESASVTVK50 within strains
observed to glycosylate these peptides support changes in glycosylation
occupancy across strains. Glycoprotein levels are denoted in red with
peptide levels denoted in blue within B to D.