3.2 Widespread m6A methylation of rice genomic mRNA with BPH infestation
The genomic mRNA m6A methylation modifications and genes expression of un-infested (Nip) and BPH-infested Nip rice (Nl-Nip) samples were compared using Nanopore Direct RNA Sequencing (DRS) and Next Generation RNA Sequencing (NGS) respectively (Supporting Information: Figure S1). Reads distribution analysis showed that all samples exhibited considerable m6A methylation enrichment within CDS and 3′-UTR regions (Supporting Information: Figure S3). At the genomic level, these unique m6A-methylated positions for each treatment were unevenly distributed across each rice chromosome (Figure 1e, Supporting Information: Table S1). Circo plots of the m6A methylome in rice genomic RNAs showed that m6A distribution density was highly consistent with the corresponding gene density on the same chromosome position in the un-infested sample (Figure 1e).