2.1. Serum panel
Sera from 32 B. canis naturally infected dogs were collected from
an outbreak occurred in a breeding kennel of Central Italy during summer
2020. The positivity to B. canis was confirmed by isolation of
the bacterium from blood cultures. Negative sera were collected from 26
healthy dogs, which were not related to B. canis outbreaks. Sera
were collected by local veterinary services according to Italian and
European regulations for animal welfare.