2.5 Statistical analysis
All statistical analysis of the data was analyzed by SPSS 27.0 and GraphPad Prism 9. All the data were expressed as mean ± standard deviation (mean ± SD). If the variance is uniform, single-factor descriptive analysis of variance (ANOVA) and least significant difference (LSD) methods were used to analyze comparisons among multiple groups. If not, the Welch test was used. P < 0.05 was considered to be statistically significant.