2.6 Sequencing and data analysis
Quality inspection was performed on the formed sequencing library, and
processing, including barcode recognition, was performed on the
high-quality circular consensus sequencing (CCS) sequence obtained. The
generated optimization CCS was clustered at the level of 97% similarity
(USEARCH, version 10.0), and its species classification was obtained
based on the sequence composition of the operational taxonomic unit
(OTU). The platforms 16S: Silva database and RDP Classifier were used to
analyze species annotation and taxonomy as well as the diversity of gut
microbiota.