\documentclass[10pt]{article}

\usepackage{fullpage}
\usepackage{setspace}
\usepackage{parskip}
\usepackage{titlesec}
\usepackage[section]{placeins}
\usepackage{xcolor}
\usepackage{breakcites}
\usepackage{lineno}
\usepackage{hyphenat}





\PassOptionsToPackage{hyphens}{url}
\usepackage[colorlinks = true,
            linkcolor = blue,
            urlcolor  = blue,
            citecolor = blue,
            anchorcolor = blue]{hyperref}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@combinedblfloats{\box\@outputbox}{\unvbox\@outputbox}{}{%
  \errmessage{\noexpand\@combinedblfloats could not be patched}%
}%
\makeatother


\usepackage{natbib}




\renewenvironment{abstract}
  {{\bfseries\noindent{\abstractname}\par\nobreak}\footnotesize}
  {\bigskip}

\titlespacing{\section}{0pt}{*3}{*1}
\titlespacing{\subsection}{0pt}{*2}{*0.5}
\titlespacing{\subsubsection}{0pt}{*1.5}{0pt}


\usepackage{authblk}


\usepackage{graphicx}
\usepackage[space]{grffile}
\usepackage{latexsym}
\usepackage{textcomp}
\usepackage{longtable}
\usepackage{tabulary}
\usepackage{booktabs,array,multirow}
\usepackage{amsfonts,amsmath,amssymb}
\providecommand\citet{\cite}
\providecommand\citep{\cite}
\providecommand\citealt{\cite}
% You can conditionalize code for latexml or normal latex using this.
\newif\iflatexml\latexmlfalse
\providecommand{\tightlist}{\setlength{\itemsep}{0pt}\setlength{\parskip}{0pt}}%

\AtBeginDocument{\DeclareGraphicsExtensions{.pdf,.PDF,.eps,.EPS,.png,.PNG,.tif,.TIF,.jpg,.JPG,.jpeg,.JPEG}}

\usepackage[utf8]{inputenc}
\usepackage[english]{babel}



\usepackage{float}








\begin{document}

\title{Inhibition of Epithelial Cell ERS Induced by AE Alleviates Epithelial
Barrier Dysfunction in LAR}



\author[1]{Feng Xiang}%
\author[1]{Hui Zhang}%
\author[1]{Ran Jing}%
\author[1]{Jie Zheng}%
\author[1]{Jianfeng Zhang}%
\author[1]{Qinxiu Zhang}%
\author[1]{Xinrong Li}%
\affil[1]{Chengdu University of Traditional Chinese Medicine}%


\vspace{-1em}



  
  \date{May 22, 2024}


\begingroup
\let\center\flushleft
\let\endcenter\endflushleft
\maketitle
\endgroup





\selectlanguage{english}
\begin{abstract}
Background: Epithelial barrier disruption is the initial pathogenesis of
various diseases. We previously reported that acupoint catgut embedding
(AE) improved tight junction proteins in rats with allergic rhinitis.
However, whether AE benefits the epithelial barrier in local allergic
rhinitis (LAR ) is unknown. Methods: In the present study, we used LAR
model rats with or without AE treatment to investigate the effect of AE
on nasal mucosal barrier function. Then, the LAR model rats were treated
with capsaicin or tunicamycin (TM) to investigate the mechanism of AE on
LAR. Results: AE ameliorated symptoms and pathological changes of nasal
mucosa in LAR rats. In addition, AE reduced inflammatory factors (IL4,
IL5, IL13) and inhibited endoplasmic reticulum stress (ERS) by reducing
substance P (SP). Finally, AE improved the epithelial barrier function
in LAR by suppressing ERS. Conclusion: the present study strongly
confirms that AE is an effective method to improve nasal barrier
function and prevent LAR barrier damage.%
\end{abstract}%



\sloppy


\textbf{Hosted file}

\verb`manuscript(3).docx`  available at \url{https://authorea.com/users/724235/articles/708312-inhibition-of-epithelial-cell-ers-induced-by-ae-alleviates-epithelial-barrier-dysfunction-in-lar}

\selectlanguage{english}
\FloatBarrier
\end{document}

