Supplementary material

Supplementary file S1 Extended M&M and R&D [PDF] Details about reasoning for using HTS data, sampling, 5S-IGS identification, pre-processing, amplicon length/GC content diversity within each sample/major type, and description of most prominent length-polymorphic patterns. Includes three supplement tables, 15 supplementary figures and two appendices. Appendix A is a summary of Supplementary file S2; appendix B includes violin and scatter plots sorted by sample and type.
Supplementary file S2 Sequence statistics and identification[XLSX] Basic information about samples, obtained HTS reads, and completely annotated lists for the 4,693 variants with an abundance of ≥ 4, as-is and summarised as Pivot tables. Uses auto-filter, auto-generating and auto-formatting functionality; up-to-date version of Excel® is recommended to properly view the file.
Supplementary file S3 EPA results [PDF] Graphical representation of the EPA analysis of 4007 5S-IGS variants with a total abundance < 25 (full results provided in Online Data Archive, folder EPA )
Supplementary file S4 Downstream in-depth analysis of 38 selected variants [XLSX] Details about selection process, including a fully annotated alignment in tabulated, graphically enhanced form.