3.6 IgE induced expression of the sphingolipid receptors in mast cells is mediated by HDAC: In vitro studies
In order to evaluate the altered sphingolipid metabolism in mast cells, the mast cells treated with IgE were evaluated for the S1P receptor expression. A significant increase in the expression of both receptors S1P1R and S1P3R (p<0.05) was observed in response to IgE treatment (Figure 5A) .
Histone deacetylation was evaluated by HDAC protein expression to see if IgE mediated sphingolipid metabolism is regulated through histone deacetylation. HDAC1 (p<0.05), HDAC2, HDAC4 and HDAC 6 (p<0.05) were found to be increased in response to IgE treatment in the mast cells cultured in vitro (Figure 5B, C) .