3.5 Altered serum Sphingosine 1-phosphate (S1P);
ceramide-1-phosphate (CIP) and serum IgE in VKC
In order to check, changes in the systemic level, the metabolites namely
S1P and C1P were estimated by ELISA. The serum C1P level was lowered in
the total VKC group compared to the control (p<0.05) and this
was seen in both R-VKC and NR-VKC (Figure 4A) . Serum SIP was
significantly higher in the non-refractory VKC compared to control
(p<0.05), while R-VKC had levels similar to control(Figure 4B) .
IgE levels were significantly increased in VKC (P=0.0007) and this was
observed both in NR-VKC (p=0.013) and in R-VKC (p=0.004) with no
difference amongst the two (Figure 4C) . The quantitative
estimation revealed a mean of 1433 ± 2061 ng/mL in control (median = 504
ng/mL), while VKC group revealed a mean of 3801±3719 ng/mL (median =
3719 ng/mL) with > 95 % case showing IgE levels more than
the median of the control.