3.6 IgE induced expression of the sphingolipid receptors in mast
cells is mediated by HDAC: In vitro studies
In order to evaluate the altered sphingolipid metabolism in mast cells,
the mast cells treated with IgE were evaluated for the S1P receptor
expression. A significant increase in the expression of both receptors
S1P1R and S1P3R (p<0.05) was observed in response to IgE
treatment (Figure 5A) .
Histone deacetylation was evaluated by HDAC protein expression to see if
IgE mediated sphingolipid metabolism is regulated through histone
deacetylation. HDAC1 (p<0.05), HDAC2, HDAC4 and HDAC 6
(p<0.05) were found to be increased in response to IgE
treatment in the mast cells cultured in vitro (Figure 5B,
C) .