Figure 4: Serum S1P, S1P and IgE in VKC estimated by ELISA
Figure 4A : Serum C1P in VKC, NR-VKC and R-VKC (Control: n= 14; VKC: 29; (NR-VKC: n= 6; R-VKC: n=23)
Figure 4B : Serum S1P in VKC, NR-VKC and R-VKC (Control: n= 9; VKC: 29; (NR-VKC: n= 6; R-VKC: n=23)
Figure 4C : Serum IgEin VKC, NR-VKC and R-VKC (Control: n= 12; VKC: 29; (NR-VKC: n= 6; R-VKC: n=23)
Figure 5: Effect of IgE on gene expression of the S1P receptors in cultured Mast cells
Figure 5A: S1P Receptors expression in Mast cells exposed to IgE
Figure 5B : Representative western blot showing protein expressions of the Histone Deacetylases, HDAC1, HDAC2, HDAC5 and HDAC6
Histone Deacetylase (HDAC) protein expression in mast cells exposed to IgE (10 and 20 ng/mL) for 16 hours compared to control in vitroas detailed in method section.
Lanes L1, 3, 5, 7- treated with 10ng/ml of IgE; L2,4,6,8- treated with 20ng/ml of IgE)
Figure 5C : Densitogram
Densitometry of the bands of the western blot was done using ImageJ software,
Y-axis: fold variation normalized to control
Student’s–t test was performed to obtain the statistical significance between control and treated *p<0.05; ** P<0.01.
The data is expressed as mean ± SD of three independent experiments