Estimation of IgE, Ceramide 1 phosphate (C1P) and Sphingosine 1
phosphate (S1P) by ELISA
The reagents, working standards and samples were prepared according to
manufacture protocol for the estimation of serum IgE, (Human IgE ELISA
Kit (ab195216); MA, USA), serum C1P (Human Ceramide-1-phosphate
-MBS2601367 ELISA kit, MyBio Source, CA, USA) and serum S1P (Human
S1P-MB5S2516132 ELISA kit, MyBio Source, CA, USA). Detection was based
on TMB substrate and the absorbance at 450nm was measured (Spinco
spectramax-M2e multimode reader, Molecular Devices, CA, USA).