Sphingolipid Enzyme gene expression by qPCR
The gene expression studies were carried out using the 50 ng/µL concentration of cDNA for assessing the sphingolipid metabolism enzymes mentioned in Supplementary Table 1.0. One µL (50 ng total RNA equivalent) in 10 µL reaction mixture was used for 50 cycles reaction in the qPCR and the fold change were analysed by ΔCq method according to Thomas(14).