2.3.1Analysis of chemical and physical properties
The total carbohydrate, uronic acid, and protein contents were
quantified by the phenol–sulfuric acid method
(Dubois, Gilles, Hamilton, Rebers, &
Smith, 1980), m-hydroxydiphenyl (Murado,
Vázquez, Montemayor, Cabo, & del Pilar González, 2005), and Bradford
method (Bradford, 1976) using glucose,
glucuronic acid, and bovine serum albumin as standards, respectively.