Fig. 6. Transplanting of fecal microbiome intervened by GP
improves intestinal inflammation. (A) The experimental protocol for FMT.
(B) Changes of body weight (n=10). (C) Histopathological changes after
HE staining. (D) Rarefaction curves of OUT quantity. (E) PCA analysis.
(F) The gut microbiota composition among experimental groups at
phylum/genus level.
The bacterial profiles of the donor mice were measured using 16S rRNA
sequencing to investigate the effects of GP on the fecal microbiome of
mice. The Simpson Diversity Index of the operational taxonomic unit
level in the DSS + GP (FMT) group was significantly higher than that of
the DSS (FMT) group, indicating that GP alters the richness and
diversity of the microbial community. Significant separations were
observed among the four groups during PCA (Fig. 6D), which is similar to
the result of hierarchical clustering. Further PCA showed that the
overall structure of the microbiota had significant differences in the
four groups (Fig.6E). The comparison of gut microflora with high
abundance at the phylum and genus levels in each group is shown in Fig.
6F. Compared with blank group, there was no significant change in the
phylum and genus levels of blank (FMT) bacteria. Compared with blank and
blank (FMT) groups, the abundance of p_ Firmicutes andp_Verrucomicrobia-g_Akkermansia in the DSS (FMT) group was
significantly lower, while the abundance of p_Verrucomicrobia ,p_Bacteroidetes, p_Proteobacteria, and
p_Firmicutes-g_Allobaculum were significantly higher. The
abundances of the above bacteria in the DSS + GP (FMT) group and the DSS
(FMT) group showed the opposite trend of change exceptp_Firmicutes- g _Oscillospira .