2.5 | ELISA
HNECs were treated with TGF-β1/TGF-β1 inhibitor or transfected with
Ad-MG53 for 72 h. The supernatant (100 µL) was used to detect the
concentration of soluble Col-a1 using ELISA kits (MeiKe, China)
following the manufacturer’s instructions. After counting the total
number of cells, the concentration of soluble collagen in each group was
calculated for 5 × 105 cells.