APPENDIX
A. Adenovirus transfection with different gradients, the expressions of
MG53/TGF-β1/Col - a1 and ZO-1 were detected by Western blot. The HNECs
were transfected adenovirus for 72h. (When Adv-MOI =100, MG53 was
significantly overexpressed and showed significant inhibitory effects on
TGF-β1, Col-a1 and ZO-1.Therefore, Adv-MOI =100 was selected as the
transfection condition).
B. The HNECs were transfected with adenovirus for 48 hours after
transfection and photographed under fluorescence
microscope(magnification 200X).
C. The HNCES were treated with different concentrations of TGF-β1
inhibitor for 72H.The inhibitory effect of MG53/TGF-β1/Smad/Col - a1 and
ZO-1 were analyzed by Western blot. (When TGF-β1 inhibitor =50n M, the
expression of MG53 /TGF-β1/ Col-a1 and ZO-1 were significant inhibited
.Therefore, TGF-β1 inhibitor =50n M was selected as the optimum
condition).