2.6 | Cell proliferation assay
HNECs were re-suspended in media containing different processing factors (TGF-β1, TGF-β1 inhibitor, or Ad-MG53), at a concentration of 5.0 × 104 cells/mL. Next, 100 μL of this cell suspension (5 × 103 cells/well) was added to each well of a 96-well plate. Cell proliferation was measured at 450 nm via live cell counting at 2, 24, 48, and 72 h after supplementation with 10 μL of CCK-8 reagent (MCE, USA).