FIG4. Si-rna transfection in HNECs with knockdown of MG53 upregulate TGF- β1/Smad pathway.
A. The cell morphology and FAM of HNECs transfected with si-rna after cells were transfected si-rna for 48h (magnification 200X). B. The expressions of MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in si-rna transfected HNEC of CRSwNP were analyzed by RT-qPCR. C and D. The expressions of MG53/TGF- β1/Smad/ Col-a1 /ZO-1 in si-rna transfected HNECs of CRSwNP were analyzed by Western blot, and quantified by densitometric analysis using ImageJ software, and data expressed as relative values against GAPDH.
Error bars are shown as means ±SEMs. Significance was calculated by using the paired Student t test. *p<0.05 and**p<0.01. ns not Significant.