3.4 | Knockdown of MG53 promoted the TGF-β1/Smad
pathway as well as the expression of ZO-1
HNECs from the specimens of the CRSwNP group were transfected by si-RNA
for MG53 knockdown to further understand the regulatory relationship
between MG53 and the TGF-β1/Smad pathway. Fluorescence after siRNA
transfection is shown in Fig. 4A. Based on the results of qRT-PCR
analysis, we observed that the expression of TGF-β1, Smad2/3, ZO-1, and
Col-a1 genes was upregulated after MG53 knockdown (Fig. 4B), consistent
with the results of western blot analysis (Fig. 4C–D).