2.5 | ELISA
HNECs were treated with TGF-β1/TGF-β1 inhibitor or transfected with Ad-MG53 for 72 h. The supernatant (100 µL) was used to detect the concentration of soluble Col-a1 using ELISA kits (MeiKe, China) following the manufacturer’s instructions. After counting the total number of cells, the concentration of soluble collagen in each group was calculated for 5 × 105 cells.