FIG4. Si-rna transfection in HNECs with knockdown of MG53
upregulate TGF- β1/Smad pathway.
A. The cell morphology and FAM of HNECs transfected with si-rna after
cells were transfected si-rna for 48h (magnification 200X). B. The
expressions of MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in si-rna transfected HNEC
of CRSwNP were analyzed by RT-qPCR. C and D. The expressions of
MG53/TGF- β1/Smad/ Col-a1 /ZO-1 in si-rna transfected HNECs of CRSwNP
were analyzed by Western blot, and quantified by densitometric analysis
using ImageJ software, and data expressed as relative values against
GAPDH.
Error bars are shown as means ±SEMs. Significance was calculated by
using the paired Student t test. *p<0.05
and**p<0.01. ns not Significant.