FIG2. The expression of MG53/TGF-β1, col-a1 and ZO-1 in HNECs of
Control、CRSsNP、CRSwNP.
A. The expression of ZO-1 (magnification 400X) in HNECs of CRSsNP,
CRSwNP and CRSwNP by immunofluorescence, and were quantified by Mean
fluorescence intensity using ImageJ software (n=3 per group). B. Western
blot analysis for MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in nasal epithelial
cells of Control, CRSsNP and CRSwNP, and GAPDH expression in total
protein extracted from HNECs. C. MG53/TGF-β1/Smad/ Col-a1 /ZO-1 were
quantified by densitometric analysis using ImageJ software, and data
were expressed as relative values against GAPDH. D. The expressions of
MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in HNECs of Control, CRSsNP and CRSwNP
were analyzed by RT-qPCR.
Error bars are shown as means ±SEMs. Significance was calculated by
using the unpaired Student t test (FIG2 A). Significance was calculated
by using the paired Student t test (FIG2 C and D). *p<0.05
and**p<0.01. ns not Significant.
FIG3. Adenovirus transfection in HNECs with
overexpression of MG53 downregulate TGF- β1/Smad pathway.
A and B. The expressions of MG53/TGF- β1/Smad/ Col-a1 /ZO-1 in
Adenovirus transfected HNECs of CRSwNP were analyzed by Western blot,
and quantified by densitometric analysis using ImageJ software, and data
expressed as relative values against GAPDH. C. The expressions of
MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in adenovirus transfected HNECs of CRSwNP
were analyzed by RT-qPCR.
Error bars are shown as means ±SEMs. Significance was calculated by
using the paired Student t test. *p<0.05
and**p<0.01. ns not Significant.