2.7 | Apoptosis assay
HNECs were treated with TGF-β1/TGF-β1 inhibitor or transfected with Ad-MG53 for 72 h. After removing the supernatant, the cells were re-suspended in PBS at a concentration of 1.0 × 106/mL, and the rate of apoptosis for each group was determined by flow cytometry.