FIG1.The expression of MG53/TGF-β1, col-a1 and ZO-1 in nasal tissues of Control、CRSsNP、CRSwNP
A. Immunohistochemistry (magnification 400X) measured the expression of Col-a1 and ZO-1 in nasal tissues of Control, CRSsNP and CRSwNP (n=5 per group). B. Immunofluorescence (magnification 800X) measured the expression of ZO-1 in nasal tissues of Control, CRSsNP and CRSwNP (n=3 per group ). C. Western blot analysis for MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in nasal tissues of Control, CRSsNP and CRSwNP, and GAPDH expression in total protein extracted from nasal tissues.D. MG53/TGF-β1/Smad2、3/ Col-a1 /ZO-1 were quantified by densitometric analysis using ImageJ software, and data were expressed as relative values against GAPDH (n=4 per group).  E. The expressions of MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in nasal tissues of Control, CRSsNP and CRSwNP were analyzed by RT-qPCR (compared with Control).
Error bars are shown as means ±SEMs. Significance was calculated by using the paired Student t test. *p<0.05 and**p<0.01.