3. RESULTS
3.1 | Expression of
MG53, TGF-β1, Smad, ZO-1, and Col-a1 was higher in CRSsNP, but lower inCRSwNP, compared with the
control
Immunohistochemical and immunofluorescent staining of nasal mucosal
specimens revealed significantly enhanced expression of ZO-1 and Col-a1
in the upper cortex in the CRSsNP group, whereas reversed patterns were
observed in the CRSwNP group (Fig. 1A). Additionally, results of
immunofluorescence showed that the AOD of ZO-1 in the CRSsNP group was
significantly higher than that in the CRSwNP group (Fig. 1B). Western
blot and qRT-PCR analyses showed that the expression of MG53, TGF-β1,
Smad2/3, ZO-1, and Col-a1 was significantly upregulated in the CRSsNP
group, but significantly downregulated in the CRSwNP group (Fig. 1C–E).