FIG2. The expression of MG53/TGF-β1, col-a1 and ZO-1 in HNECs of Control、CRSsNP、CRSwNP.
A. The expression of ZO-1 (magnification 400X) in HNECs of CRSsNP, CRSwNP and CRSwNP by immunofluorescence, and were quantified by Mean fluorescence intensity using ImageJ software (n=3 per group). B. Western blot analysis for MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in nasal epithelial cells of Control, CRSsNP and CRSwNP, and GAPDH expression in total protein extracted from HNECs. C. MG53/TGF-β1/Smad/ Col-a1 /ZO-1 were quantified by densitometric analysis using ImageJ software, and data were expressed as relative values against GAPDH. D. The expressions of MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in HNECs of Control, CRSsNP and CRSwNP were analyzed by RT-qPCR.
Error bars are shown as means ±SEMs. Significance was calculated by using the unpaired Student t test (FIG2 A). Significance was calculated by using the paired Student t test (FIG2 C and D). *p<0.05 and**p<0.01. ns not Significant.
FIG3. Adenovirus transfection in HNECs with overexpression of MG53 downregulate TGF- β1/Smad pathway.
A and B. The expressions of MG53/TGF- β1/Smad/ Col-a1 /ZO-1 in Adenovirus transfected HNECs of CRSwNP were analyzed by Western blot, and quantified by densitometric analysis using ImageJ software, and data expressed as relative values against GAPDH. C. The expressions of MG53/TGF-β1/Smad/ Col-a1 /ZO-1 in adenovirus transfected HNECs of CRSwNP were analyzed by RT-qPCR.
Error bars are shown as means ±SEMs. Significance was calculated by using the paired Student t test. *p<0.05 and**p<0.01. ns not Significant.