Bupropion effect on concentration of target cytokines in PBMCs culture
To discover the alterations in pro-inflammatory cytokines profile in response to bupropion, LPS-PHA treated peripheral blood mononuclear cells get treated with different concentrations of bupropion. As depicted in graphs (Fig. 1A-C), bupropion administration significantly decreased the secretion of pro-inflammatory cytokines including TNF-ɑ, IL-1ß, and IL-17 levels in media assessed by ELISA. We further evaluated the levels of anti-inflammatory cytokine IL-10; bupropion treatment resulted in a significant increase in IL-10 concentration in the test cell culture (Fig.1D).