Bupropion effect on gene expression of target cytokines in PBMCs
culture
In search of the inflammatory response to bupropion treatment at the
gene expression level, we assessed the relative expression of
pro-inflammatory interleukins performing real-time PCR. Interestingly,
mRNA levels of pro-inflammatory cytokines including TNF-ɑ, IL-1ß, and
IL-17 were upregulated in cultures following bupropion incubation (Fig.
2A-C). Bupropion treatment also increased mRNA levels of IL-10 compared
to the untreated culture (Fig. 2D).