Bupropion effect on concentration of target cytokines in PBMCs
culture
To discover the alterations in pro-inflammatory cytokines profile in
response to bupropion, LPS-PHA treated peripheral blood mononuclear
cells get treated with different concentrations of bupropion. As
depicted in graphs (Fig. 1A-C), bupropion administration significantly
decreased the secretion of pro-inflammatory cytokines including TNF-ɑ,
IL-1ß, and IL-17 levels in media assessed by ELISA. We further evaluated
the levels of anti-inflammatory cytokine IL-10; bupropion treatment
resulted in a significant increase in IL-10 concentration in the test
cell culture (Fig.1D).