FIGURE LEGENDS
Figure 1. Bupropion effect on the secretion of the pro-/anti-inflammatory cytokines. Bupropion treatment significantly suppressed (A) IL-1ß, (B) TNF-ɑ, and (C) IL-17 concentration while increased the (D) IL-10 levels in PBMCs media. The results are presented as mean ± S.D, ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ P < 0.0001. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by the Sidak’s multiple comparisons test.
Figure 2. Bupropion effect on gene expression of target cytokines gene. Bupropion significantly enhanced the gene transcription of (A) IL-1ß, (B) TNF-ɑ, (C) IL-17, and (D) IL-10. Fold changes are calculated with the 2−∆∆Ct method. The results are presented as mean ± S.D, ∗ p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗ P < 0.0001. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by the Sidak’s multiple comparisons test.
Figure 3. Bupropion effect on gene expression of TLRs. Treatment of LPS+PHA-stimulated cells with bupropion promoted the mRNA expression of (A) TLR2 and (B) TLR4. Fold changes are calculated with the 2−∆∆Ct method. The results are presented as mean ± S.D, ∗ p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗ P < 0.0001. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by the Sidak’s multiple comparisons test.
Figure 4. Bupropion effect on gene expression of JAK2 /STAT3. Treatment of LPS+PHA-stimulated cells with bupropion promoted the mRNA levels of (A) JAK2 and (B) STAT3. Fold changes are calculated with the 2−∆∆Ct method. The results are presented as mean ± S.D, ∗ p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗ P < 0.0001. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by the Sidak’s multiple comparisons test.