Cytokine Array and Enzyme-Linked Immunosorbent Assay (ELISA)
After 72 h co-treatment of cells with PHA+ LPS and bupropion, the plate was centrifuged for 10 min, the supernatant removed, and get aliquoted. Cytokines concentration of IL-1β, IL-17, TNFα, and IL-10 were determined using ELISA kits (R&D Systems, US) according to the manufacturer’s protocol. Optical density measurements took at 450 nm.