Tropisetron effect on the gene expression of JAK2 and STAT3
Several cytokines signal through the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway which is involved in the development and modulation of immune responses. Among the human JAK four family members, JAK2 is the most common kinase activated by two-thirds of ligands. Following activation, STAT3 can promote the production of both inflammatory cytokines like IL-17 and IL-6 and anti-inflammatory cytokines (like TGF-β and IL-10) 24. We focused on this kinase and related transcription factor to elucidate the molecular signaling pathway that might account for the bupropion immunomodulatory actions. Bupropion treatment led to a considerable rise in gene expression of JAK2 at 50 and 100 concentrations (Fig. 4A). We also found out that LPS-treated PBMCs significantly enhanced STAT3 gene expression in the presence of bupropion (Fig. 4B).