Bupropion effect on gene expression of target cytokines in PBMCs culture
In search of the inflammatory response to bupropion treatment at the gene expression level, we assessed the relative expression of pro-inflammatory interleukins performing real-time PCR. Interestingly, mRNA levels of pro-inflammatory cytokines including TNF-ɑ, IL-1ß, and IL-17 were upregulated in cultures following bupropion incubation (Fig. 2A-C). Bupropion treatment also increased mRNA levels of IL-10 compared to the untreated culture (Fig. 2D).