FIGURE LEGENDS
Figure 1. Bupropion effect on the secretion of the
pro-/anti-inflammatory cytokines. Bupropion treatment significantly
suppressed (A) IL-1ß, (B) TNF-ɑ, and (C) IL-17 concentration while
increased the (D) IL-10 levels in PBMCs media. The results are presented
as mean ± S.D, ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ P
< 0.0001. Statistical analysis was performed by one-way
analysis of variance (ANOVA) followed by the Sidak’s multiple
comparisons test.
Figure 2. Bupropion effect on gene expression of target cytokines gene.
Bupropion significantly enhanced the gene transcription of (A) IL-1ß,
(B) TNF-ɑ, (C) IL-17, and (D) IL-10. Fold changes are calculated with
the 2−∆∆Ct method. The results are presented as mean ±
S.D, ∗ p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗ P
< 0.0001. Statistical analysis was performed by one-way
analysis of variance (ANOVA) followed by the Sidak’s multiple
comparisons test.
Figure 3. Bupropion effect on gene expression of TLRs. Treatment of
LPS+PHA-stimulated cells with bupropion promoted the mRNA expression of
(A) TLR2 and (B) TLR4. Fold changes are calculated with the
2−∆∆Ct method. The results are presented as mean ±
S.D, ∗ p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗ P
< 0.0001. Statistical analysis was performed by one-way
analysis of variance (ANOVA) followed by the Sidak’s multiple
comparisons test.
Figure 4. Bupropion effect on gene expression of JAK2 /STAT3. Treatment
of LPS+PHA-stimulated cells with bupropion promoted the mRNA levels of
(A) JAK2 and (B) STAT3. Fold changes are calculated with the
2−∆∆Ct method. The results are presented as mean ±
S.D, ∗ p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗ P
< 0.0001. Statistical analysis was performed by one-way
analysis of variance (ANOVA) followed by the Sidak’s multiple
comparisons test.