Yeast two hybrid and bimolecular fluorescence complementation
assay
For Y2H analysis, all cDNAs of SlUVI4 and SlCCS52s were
cloned into pDEST-GADT7 and pDEST-GBKT7 (Rossignol, Collier, Bush, Shaw,
& Doonan, 2007) and assays were performed following the user manual of
Matchmaker Gold Yeast Two-Hybrid System (Clontech Laboratories, Inc.).
For BiFC analysis, SlUVI4 cDNA was cloned into the destination
vector pSPYNE-35S GW and cDNAs of SlCCS52 genes were cloned into
the destination vector pSPYCE-35S GW (Walter et al., 2004). These
vectors were transiently transformed into onion cells via Agrobacterium
tumefacien strain GV3101 together with gene silencing inhibitor P19
(Schutze, Harter, & Chaban, 2009; W. Sun, Cao, Li, Zhao, & Zhang,
2007). GFP signals were detected using Nikon fluorescence microscope
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