Gene expression analysis
Total RNA from hypocotyls was extracted by RNAisoPlus (TaKaRa, Otsu, Japan, cat. #) following the manufacturer’s instruction. The first strand complementary DNA (cDNA) synthesis using 2μg total RNA was performed following instructions provided with HiScript III RT SuperMix kit with gDNA wiper (Vazyme, cat. #R323-01) for qPCR analysis and HiScript III 1st Strand cDNA Synthesis Kit with gDNA wiper (Vazyme, cat. #R312-01) for full-length gene cloning. One microliter and 0.2μl of cDNA templates were used the amplification of SlCBL1 gene by RT-PCR to determine the quality of cDNA and qPCR analysis, respectively.SlCBL1 was used as a reference gene for qRT-PCR data normalization (Pombo et al., 2014). All primers were designed by on-line tools (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) and listed in Supplementary Table 1.