Identification of APC/C regulators in tomato
We isolated tomato homologs of APC/C regulators through BLAST analysis
in the website (www.solgenomics.net). Tomato has only one UVI4homologous gene Solyc10g080400 in the genome referred asSlUVI4 , which is different with other plant species such as
Arabidopsis, rice, maize, strawberry, etc. We further identifiedUVI4 homologous genes in
other Solanaceae species, and
revealed that pepper, potato and eggplant have one UVI4 homolog,
and tobacco and petunia have four and three homologs in the draft
genome, respectively
(Supplementary Figure S1A).
Similar to Arabidopsis, tomato has three CCS52 homologous genes
referred as SlCCS52s including Solyc08g080080(SlCCS52A1 , previously named as SlCCS52A ),Solyc12g056490 (SlCCS52A2 ) and Solyc06g043150(SlCCS52B ) and four CDC20 homologous genes referred asSlCDC20s including Solyc03g096870 (SlCDC20.1 ),Solyc08g005420 (SlCDC20.2 ), Solyc06g072830(SlCDC20.3 ) and Solyc03g095210 (SlCDC20.4 )
(Supplementary Figure S1B). SlCCS52A2 is newly isolated in tomato
genome, and its amino acid sequence has 83.05% and 57.75% identity
with SlCCS52A1 and SlCCS52B, respectively (Figure S2). We analyzed
subcellular localizations of SlUVI4 and SlCCS52 proteins, and revealed
that all of them have nuclear localization (Figure S3). SlCCS52A1 also
has strong plasma membrane localization, and SlCCS52B has weak
cytoplasmatic localization. We further tested the interactions between
SlUVI4 and SlCCS52 proteins, and both yeast-two hybrid and
bimolecular fluorescence
complementation assay (BIFC) revealed that SlUVI4 could interact with
SlCCS52A1 and SlCCS52A2, but not with SlCCS52B (Figure 2). All these
data suggest that SlUVI4 and SlCCS52 genes might have
similar functions as those in Arabidopsis.