3. Results

32 rats in the 4 groups in our study were followed 24 hours after the drug and fluid administrations. All the animals had free access to food and water. After 24 hours, mice were sacrificed by cervical dislocation. No rats were lost during this period. Heart, liver and kidneys were dissected and subjected to histopathological examination. Group 1 was determined as the control group. Histopathological scoring results were obtained according to microscopic examination of liver tissues. Figure 1 represents the microscopic views of the hepatic tissues. Comparison of histopathological scores of liver tissues between groups are shown in figure 2. There was no statistically significant difference between 1st (control) and 2nd (ILE) groups in terms of histopathological scoring. 3rd (PIO) and 4th (PIO+ILE) groups showed a statistically significant increase in histopathological scoring compared to 1st (control) group in terms of increased tissue damage (p <0.001). There was no statistical difference between the 3rd (PIO) and 4th (PIO+ILE) groups. Histopathological scoring results obtained by microscopic examination of cardiac tissues are shown in Figure 3. Histopathological scores were higher in group 3 and 4 than in other groups but there was no statistically significant difference between the groups in terms of histopathological scoring. Figure 4 shows the comparisons of the results in terms of cardiac histopathological scoring. Histopathological scoring results obtained according to microscopic examination of kidney tissues are shown in Figure 5. The 2nd (ILE), 3rd (PIO) and 4th (PIO+ILE) groups showed a statistically significant difference in terms of histopathological scoring compared to the 1st group (control) (p <0.001). There was no statistically significant difference between 2nd (ILE), 3rd (PIO) and 4th (PIO+ILE) groups. Figure 6 represents the comparisons among the groups.