5. Limitations

This is an experimental animal study and small number of subjects is the main limitation due to ethics considerations. Although we had used pioglitazone and ILE at doses used in the previous animal studies, they may not be effective in order to demonstrate our hypothesis. We had evaluated the tissue effects after 24 hours. This time period may not be enough for histopathological changes to be observed especially for the heart. As also mentioned in the previous studies, ILE administration may prevent biochemical parameters from being measured accurately. Therefore, we could not say anything about molecular based changes in the blood in terms of organ functions.