Study area and puma capture
Our study was conducted in two regions in the Rocky Mountains in Colorado separated by ~500 km but at similar elevations and with similar puma densities (Lewis et al. 2015), vegetative and landscape attributes, yet with differing degrees of urbanization (see Fig. S10 and Lewis et al. 2015). In the treatment region in the Uncompahgre Plateau on the Western Slope of Colorado, blood samples were taken from 114 individuals (2004-2011: 50 females & 33 males, 2012-2014: 21 females & 19 males) and monitored intensively (e.g., GPS collars) until their death or the end of the study in 2014. In the stable management region in the Front Range of Colorado, blood samples were taken from 56 individuals from 2005-2014 (2005-2011: 11 females & 5 males, 2012-2014: 21 females & 19 males). Captured pumas were anesthetized with established sedative and tranquilizer protocols (Logan 2012) and released after blood, serum, and oral swabs were collected. Animal sex, age, and capture location were recorded. See Fountain-Jones et al. (2019) for sample storage, FIVpco DNA extraction and sequencing details. In brief, for samples that were qPCR positive for FIVpco, the complete ORFA and pol gene regions were isolated using a nested PCR protocol (Fountain-Jones et al. 2019). Recombination was removed and the genes were concatenated together. See Table S1 for a summary of the sequence data and a comparison of study area size, host mortality, and host genetic diversity between regions.