Each patient was subjected to the following:
1-Full detailed history and examination to exclude any comorbid condition that may affect the result of the study.
2-Full detailed allergic history and clinical examination of the respiratory system
3-Chest x ray was done to exclude other lung pathology.
4- Pulmonary function tests with special emphasis on FEV1, FEV1/FVC using SPIROMETRICS
5- Total serum IgE was done for each patient using enzyme-linked immunosorbent assay (ELISA).
6- Skin prick testing to common allergens.
7-Asthma severity score was calculated according to GINA, 2020. [7]
8- Serum zonulin level detection by ELISA