Sample size:
Assuming that serum zonulin level in case group versus control group is (11.3 ± 3.7 ng/ml) and (9.3 ± 3.3 ng/ml) respectively with confidence level 95% and power 80%. So the sample size is 96 (48 in every group)
They will be divided into 2 groups.
Group 1 (Control group): includes 48 healthy controls who fulfilled the same exclusion criteria as the patients, and never had asthma or eczema.
Group 2 (Asthmatic patients): includes 48 adult (≥18 years old) subjects with allergic asthma according to Global Initiative for asthma consensus report (GINA), 2020 . [7]