Serum level of total IgE:
Quantitative measurement of serum level of total IgE was done using a
commercially available sandwich enzyme-linked immunosorbent assay
(ELISA) Kit supplied by Chemux Bioscience, Inc. (CA, USA, Cat
No.10602) according to the manufacturer’s instructions. The results
were expressed in IU/mL. The total IgE level in a normal, allergy-free
adult is less than 150 IU/ml of serum. The minimum detectable
concentration of IgE by this assay is estimated to be 5.0 IU / ml. The
Absorbance of standards and samples were measured at 450 nm using a
microtiter plate ELISA reader (Biotek, USA).
Measurement of serumzonulin level:
Quantitative measurement of serum level of zonulin was done using a
commercially available sandwich enzyme-linked immunosorbent assay
(ELISA) Kit supplied by MyBiosource, Inc. (USA, Cat No:
MBS774026) according to the manufacturer’s instructions. The results
were expressed in ng/ml. Absorbance of standards and samples were
measured at 450 nm using a microtiter plate ELISA reader(Biotek, USA). The kit detection range was 20ng/ml - 800ng/ml.